摘要
采集广东省某猪场腹泻仔猪的小肠病料,通过在Vero细胞上培养,RT-PCR及间接免疫荧光检测,电镜观察等方法鉴定,确定分离到一株PEDV毒株,并命名为PEDV-GD12。通过对PEDV M基因的克隆测序以及进化分析,显示所分离到的毒株近年来流行的毒株同源性很高,处在同一分支上。
A porcine epidemic diarrhea virus （PEDV） designated PEDV-GD12 was isolated from the small intestine feces of diarrheaed piglets in Guangdong province and cultured in Veto cell.The isolated virus was identified by RT-PCR,immunofluorescence,electronic microscope etc.The M gene of PEDV was amplified and sequenced and namecl by PEDV-GD 12.Phytogenetic analysis showed that the isolated strain shares a high homology with the pandemic strains in recent years and they are clustered into the same branch.
出处
《中国兽医杂志》
CAS
北大核心
2013年第11期22-25,共4页
Chinese Journal of Veterinary Medicine
基金
现代农业产业技术体系建设专项资金资助(CARS-36)
关键词
PEDV
分离鉴定
M基因
进化分析
PEDV
isolation and identification
M gene
phylogenetic analysis.
