摘要
目的:探讨荞麦糠皮提取物(EBC)对2型糖尿病(T2DM)大鼠心肌损伤的保护作用,并阐明其保护作用机制。方法:采用小剂量链脲佐菌素(STZ)加高糖高脂饲料喂养的方法建立大鼠T2DM模型。建模成功的23只T2DM大鼠分为模型对照组(n=8)、EBC治疗组(n=7)和贝那普利治疗组(n=8),另取7只SD大鼠作为正常对照组。EBC治疗组和贝那普利治疗组大鼠分别给予EBC(200mg·kg-1·d-1)和贝那普利(4mg·kg-1·d-1)灌胃;正常对照组和模型对照组大鼠分别给予纯水(10mL·kg-1·d-1)。灌胃给药8周后,检测各组大鼠体质量(BW)、心脏质量指数(HWI)、空腹血糖(FBG)水平和左室收缩压(LVSP)、左心室舒张期末压(LVEDP)、心率(HR)、左室内压最大上升下降速率(±dp/dtmax);光镜下观察各组大鼠心肌组织形态学改变。结果:与正常对照组比较,模型对照组大鼠FBG、HWI、LVEDP升高(P<0.01),BW、LVSP、HR和±dp/dtmax下降(P<0.05或P<0.01);与模型对照组比较,EBC治疗组和贝那普利治疗组大鼠BW、LVSP、HR、±dp/dtmax明显升高(P<0.05或P<0.01);而HWI、FBG和LVEDP显著降低(P<0.05或P<0.01);EBC治疗组和贝那普利治疗组大鼠心肌组织形态得到改善。结论:EBC可以改善T2DM大鼠心脏组织学形态,其机制可能为通过上调LVSP、HR和±dp/dtmax、下调LVEDP而改善心功能。
Objective To study the protective effect of extract of buckwheat chaff （EBC mellitus） on myocardial injury in type 2 diabetes mellitus （T2DM） rats and to disscuss its mechanism. Methods T2DM rat models were established by injecting streptozotocin （STZ） and given high-fat diet. 23 T2DM rats were divided into model control group （n=8）, EBC treatment group （n=7）, and benazepril treatment group （n=8）. Another 7 SD rats were selected as normal control group. The rats in EBC treatment group and benazepril treatment group were administered with EBC （200mg·kg^-1·d^-1） and benazepril （4mg·kg^-1·d^-1）orally. While the rats in other two groups were administered with normal water （10 mL·kg^-1·d^-1） instead. The levels of fasting blood glucose （FBG）, body weight （BW）, cardiac index （HWI）, heart rate （HR）, left ventricular systolic blood pressure （LVSP）, left ventricular diastolic pressure（LVEDP） and ±dp/dtmax of rats were measured 8 weeks after treatment in various groups. The morphological changes of myocardium tissue of rats were observed with light microscope. Results Compared with normal control group, the FBG, HWI, LVEDP of the rats in model group were increased （P〈0.01）, and the BW, LVSP, HR, and ±dp/dtmax in model control groups were decreased（P〈0.05 or P〈 0.01）. Compared with model control group, the BW, LVSP, HR, and ±dp/dtmax were increased and the HWI, FBG and LVEDP of the rats in EBC and benazepril treatment groups were decreased（P〈0.05 or P〈0.01）. The morphology of myocardium tissue of the rats in EBC treatment and benazpril treatment groups were improved. Conclusion EBC can improve the histomorphology of myocardium tissue of the T2DM rats, and its mechanism may be related to up-regulating the LVSP, HR and ±dp/dtmax and down-regulating the LVEDP.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2013年第6期1215-1218,1315,共5页
Journal of Jilin University：Medicine Edition
基金
河北省科技厅科技计划项目资助课题(122077175D)
河北省自然科学基金资助课题(C2010001795)
河北联合大学科学研究基金资助课题(Z2001128)
关键词
荞麦
糠皮提取物
2型糖尿病
血糖
心功能
buckwheat
extract of buckwheat chaff
type 2 diabetes mellitus
glucose
cardiac function
