摘要
【目的】研究木瓜蛋白酶解法提取林蛙卵多糖的最佳工艺,测定提取的林蛙卵多糖的抗氧化活性。【方法】以林蛙卵为材料,通过单因素试验和正交试验分析料(g)液(mL)比、木瓜蛋白酶加酶量、pH值和酶解时间对多糖提取率的影响,确定林蛙卵多糖的木瓜蛋白酶法最佳提取工艺;应用1,1-二苯基-2-三硝基苯肼(DPPH)法测定林蛙卵多糖的抗氧化活性。【结果】木瓜蛋白酶提取林蛙卵多糖的最佳条件为:料(g)液(mL)比1∶20,酶添加量为每g样品70mg,pH 6.0,酶解时间2.5h,在此条件下,林蛙卵多糖提取率为2.67%。林蛙卵多糖对DPPH自由基的半数清除率为1.81mg/mL。【结论】木瓜蛋白酶法对林蛙卵多糖的提取效果较好,林蛙卵多糖具有明显的抗氧化作用。
【Objective】This study focused on the optimization of polysaccharides extraction from brown frog egg with papain enzymatic hydrolysis and the determination of antioxidant activity of extracted polysaccharides.【Method】Using brown frog egg as material,the effects of material to liquid ratio,enzyme concentration,pH and hydrolysis time on the extraction rate of polysaccharides were studied through the single factor experiments and orthogonal test analysis.The optimum extraction conditions were determined and the antioxidant activity of extracted polysaccharides was measured by DPPH method.【Result】The obtained optimum conditions were：material to liquid ratio was 1 g∶20 mL,the enzyme amount was 70 mg/g,pH value was 6.0 and hydrolysis time was 2.5 h.Under the optimal conditions,the extraction yield of polysaccharides from brown frog egg was 2.67%,and the half clearance rate on DPPH radicals was 1.81 mg/mL.【Conclusion】Extraction of polysaccharides from brown frog egg with papain enzymatic hydrolysis was efficient and the extracted polysaccharides had significant antioxidant activity.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2013年第12期149-154,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
吉林省科技发展计划项目(08SYS-097)
关键词
木瓜蛋白酶
林蛙卵
多糖
工艺优化
抗氧化
papain
brown frog egg
polysaccharides
optimum extracting technology
antioxidant activity
