摘要
采用单因素试验和响应曲面法对酸奶中嗜热链球菌H1的最适生长条件进行了研究和优化.根据回归分析得到的最适培养条件为:温度41.02℃,pH5.74,接种量0.31mL·50mL-1,盐浓度0.23%,此时菌株的光密度OD600为1.176.经实验验证,在该条件下菌株光密度OD600为1.162,其相对误差小于2%.
The growth conditions of S .thermophilus H1 were studied by single factor experiment and response surface methodology . The results showed that the optimal parameters for the growth conditions as follows ：the temperature was 41 .02℃ ,the pH was value 5 .74 ,the inoculum size was 0 .31mL · 50mL -1 ,the salt concentration was 0 .23% ,and the OD600 of strain HI was 1 . 176 .Under the optimal conditions the OD600 of strain HI was 1 .162 ,The relative error was bel-lo w 2% .
出处
《山东理工大学学报（自然科学版）》
CAS
2013年第6期26-31,共6页
Journal of Shandong University of Technology:Natural Science Edition
基金
国家级大学生创新创业训练计划项目(201210446007)
山东省自然科学基金资助项目(ZR2010CL014)
山东省高等学校科技计划项目(J12LE02)
曲阜师范大学青年基金资助项目(XJ201107
XJ201001)
关键词
嗜热链球菌
响应曲面法
生长条件
优化
S. thermophilus
growth condition
response surface methodology
optimization
