摘要
目的结合SysmexUF一1000i尿流式分析仪和盈东URISCANPROⅡ尿干化学分析仪的自动化检测结果，通过对实验数据的分析，建立适合本实验室的自动化尿液分析的复检规则。方法（1）收集该院2013年1～3月共1000份尿液标本，所有标本都使用SysmexUF-1000i尿流式分析分析仪、URISCANPROⅡ尿干化学分析仪和尿沉渣显微镜检测。（2）以镜检结果为判断标准，统计本实验室制定复检规则的真阳性率、假阳性率、真阴性率、假阴性率（漏诊率）及复检率。结果根据本科室建立的63条复检规则对检测结果进行统计分析，真阳性率为45．0％（450／1000），假阳性率为18．9％（189／1000），真阴性率为31．9％（319／1000），假阴性率（漏诊率）为4．2％（42／1000），复检率为24．8％（248／1000），对其中的42份假阴性标本进行分析，红细胞的镜检结果都小于或等于12个／μL，白细胞的镜检结果都小于或等于17个／μL，无1例病理管型假阴性，所以对严重肾功能异常患者不会造成漏诊。结论针对SysmexUF-1000i尿流式分析仪和盈东URISCANPROⅡ尿干化学分析仪的自动化检测结果，本实验室制定的复检规则能够有效地筛选出需要复检的标本，既提高了检测效率，减轻了劳动强度，又能防止漏检和误检，是理想复检方案。
Objective Combined with the results of Sysmex UF-1000i urine sediment flow cytometry analysis and URISCAN PRO Ⅱ urine strip chemistry analysis,the review criteria were established for automated urine analysis through experimental data analysis in our lab. Methods （1）1000 urine samples were collected from the Affiliated Tong Ji Hospital of Tong Ji University during January and March 2013. All of them were analyzed by Sysmex UF-1000i urine sediment flow cytometer, URISCAN PRO Ⅱ urine dry chemical analyzer and microscopic examination. （2） Judgment on the results of microscopic examination standard, the true positive rate, false positive rate, true negative rate,false negative rate and review rate of the review criteria were calculated in our lab. Results According to 63 rules of the review criteria in our lab, the statistical results showed the true positive rate, false positive rate, true negative rate, false negative rate and review rate were 45.0 %. 18.9 %. 31.9 %,4.2 % and 24.8 %, respectively. Analyzed the 42 false negative samples,the microscopic examination results of RBC and WBC were less than 12.1 cells/μL, and 17.1 cells/μL, respectively. The result of pathological CAST was no omission. Therefore,the review criteria could avoid the missed diagnosis of the patients with severe renal disease. Conclusion The microscopy review criteria established for the automated urine analysis could effectively detect abnormal urine samples, improve the efficiency and prevent missed and false diagnosis. It was an ideal criteria.
出处
《检验医学与临床》
CAS
2013年第A02期3-5,共3页
Laboratory Medicine and Clinic
