摘要
目的:探讨基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)及其抑制因子(tissue inhibitor of matrix metalloproteinase-2,TIMP-2)在胎膜早破合并绒毛膜羊膜炎患者胎膜组织中的表达及意义。方法:收集分娩产妇124例,分为足月胎膜早破组45例,早产胎膜早破组40例,足月未临产剖宫产39例为对照组,分娩后根据病理结果分为合并绒毛膜羊膜炎及非合并绒毛膜羊膜炎。采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(streptavidin-perosidase,SP)法检测胎膜组织中MMP-2、TIMP-2的蛋白表达。结果:胎膜早破组患者胎膜组织MMP-2的表达明显高于对照组(P<0.05),TIMP-2的表达明显低于对照组(P<0.05),足月胎膜早破组和早产胎膜早破组之间差异无统计学意义(P>0.05);胎膜早破合并绒毛膜羊膜炎患者胎膜组织MMP-2的表达高于未合并绒毛膜羊膜炎患者(P<0.05),TIMP-2的表达显著低于未合并绒毛膜羊膜炎患者(P<0.05)。结论:胎膜早破合并绒毛膜羊膜炎的发生可能与MMP-2和TIMP-2的表达异常相关。
Objective： To investigate the expression and significantce of matrix metalloproteinase-2 （MMP-2） and tissue inhibitory factor-2 （TIMP-2） in patients with premature rupture of membranes （PROM） combined chorioamnionitis. Methods： A total of 124 pregnant women were selected and divided into term PROM group （45 cases）, preterm PROM group （40 cases） and the control group （39 full term women with cesarean delivery）. Pregnant women were divided into combined chorioamnionitis group or not combined chorioamnionitis group according to pathological result after delivery. The protein levels of MMP- 2 and TIMP- 2 in each group were detected by immunohistochemistry. Results： The expression of MMP-2 in the patients with PROM was significantly higher than that of control group （P%0.05）, the ex- pression of TIMP-2 was significantly lower than that of control group （P0. 05）. The expression of MMP-2 in patients with premature rupture of membranes combined chorionic amniotic membrane inflam- mation was significantly higher than that of unincorporated chorionic amniotic membrane inflammation pa- tients （P..0.05）, the expression of TIMP-2 was significantly lower than unincorporated chorionic amniot-
出处
《海南医学院学报》
CAS
2014年第2期169-171,176,共4页
Journal of Hainan Medical University
基金
深圳市宝安区科技计划项目(2013202)~~
