摘要
目的 :建立测定复方替硝唑搽剂中替硝唑和水杨酸含量的高效液相色谱法。方法 :采用反相ODS色谱柱 ,甲醇 - 0 0 2mol·L-1KH2 PO4 溶液 (5 0∶5 0 )为流动相 ,检测波长为 315nm。结果 :替硝唑和水杨酸的线性范围分别在5 0～ 35 0 μg·mL-1(r=0 9997) ,40 0～ 16 0 0 μg·mL-1(r =0 9997) ;加样回收率分别为 10 0 6 % (RSD =0 6 9% )及 10 0 6 % (RSD =0 79% ) ;日内RSD为 0 16 %～ 1 2 %和 0 6 1%～ 1 2 % ,n =12 ,日间RSD为 0 2 4%～ 1 7%和0 2 %～ 1 1% ,n =4。结论 :HPLC法可用于复方替硝唑搽剂的含量测定 ,方法准确、灵敏 。
Objective:To establish a HPLC method for determination of tinidazole and salicylic acid in its liniment.Method:Techsphere ODS column was used as the stationary phase and methanol-0 02 mol·L -1 KH 2PO 4(50∶50,adjust pH to 3 with 10% phosphate acid )was used as mobile phase,the detective wavelength was 315 nm.Results:The linear ranges of tinidazole and salicylic acid were 5～35 μg·mL -1 (r=0 999 7) and 40～160 μg·mL -1 (r=0 9997),respectively;The average add sample recovery were 100 6% (RSD=0 69%) and 100 6% (RSD=0 79);The RSD of intra-day were 0 16%～1 2% and 0 61%～1 2%,n=12.The RSD of inter-day were 0 24%～1 7% and 0 2%～1 1%,n=4.Conclusion:The method is accurate,sensitive for the analysis of compound tinidazole liniment.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2000年第5期329-331,共3页
Chinese Journal of Pharmaceutical Analysis
