摘要
多聚半乳糖醛酸酶抑制蛋白(PGIP)是存在于多种植物细胞壁中的一种糖蛋白,它能专一性识别真菌多聚半乳糖醛酸酶并抑制其活性,并能在一定程度上抑制真菌对植物细胞壁的降解。该蛋白在植物抵制真菌病害的发生中发挥着重要作用。文中利用PCR技术,从抗病能力较强的云南野生中华猕猴桃中成功地克隆了多聚半乳糖醛酸酶抑制蛋白基因(PGIP)的完整开放阅框架,并对其进行了序列和BLAST比对分析,此外,还通过生物信息学分析,对该基因氨基酸序列进行了结构域分析和蛋白质高级结构预测。结果表明:所得的PGIP基因996 bp为一个完整的开放阅读框,编码332个氨基酸;该片段与其它植物中的PGIP有很高的同源性,其与美味猕猴桃的同源性最高(达99%),与美洲李、越桔灌蓝莓和葡萄的PGIP基因的同源性分别为96%、78%、72%。该基因的成功克隆与文中的分析结果,为后续的基因功能确证及猕猴桃品种改良打下了基础。
Polygalacturonase-irthibiting protein （PGIP） is a glycoprotein existing in the cell wall of many plants. It can recognize fungal polygalacturonase specifically and then control its activity. It can prevented fungi from degrading plant cell wall at a certain extent. The protein plays an important role in plant resistance to fungal diseases. The complete open reading frame ofPGIP gene was cloned from wild Actinidia chinensis in Yunnan with relatively strong disease-resistance by PCR techniques. Then sequences analysis and BLAST analysis were carried on. In addition, structure domains of the amino acid sequences were analyzed and protein structure was predicted by bioinformatic analysis. The results show that the PGIP fragment represents an open reading frame of 996 bp encoding 333 amino acids, it shows high homology with the PGIP sequences in other plants, homologous rate between A. deliciosa and target gene is up to 99%, and homologous rates between the sequences from Prunus americana, Vaccinium corymbosum, Vitis vinifera and the target sequence are 96%, 78% and 72%, respectively. Successfully cloning and analysis result of the gene laid good foundations for subsequent gene function corroboration and improvement of kiwi fruit cultivars.
出处
《经济林研究》
北大核心
2013年第4期73-77,共5页
Non-wood Forest Research
基金
国家林业局948项目(2012-4-62)
云南省省院省校教育合作咨询共建重点学科资助项目(211015)
关键词
中华猕猴桃
PGIP基因
克隆
分析
Actinidia chinensis
polygalacturonase-inhibiting protein （PGIP） gene
clone
analysis
