摘要
目的研究α甲酰基辅酶A消旋酶(P504S)、P63基因、高分子量角蛋白(CK5/6、34βE12)联合标记在不同性质的前列腺穿刺活检标本中的表达特点,探讨它们在前列腺良、恶性疾病诊断及鉴别诊断中的意义。方法收集芜湖市第一人民医院病理科2008年1月—2012年12月前列腺穿刺活检标本50例,其中前列腺癌30例,前列腺增生性病变20例,采用免疫组织化学染色(S-P法)检测标本的P504S、P63、CK5/6、34βE12表达。结果 P504S在前列腺癌中呈阳性,前列腺增生性病变中呈阴性,差异有统计学意义(P<0.05);而P63、CK5/6、34βE12在前列腺癌中基底细胞表达缺失,在前列腺增生性病变中基底细胞阳性表达,差异有统计学意义(P<0.05)。结论P504S、P63、CK5/6、34βE12的联合应用对前列腺穿刺标本的诊断和鉴别诊断有良好的应用价值。
Objective To study the expression and diagnostic utility ofa-Methylacyl-eoenzyme A racemase （AMACR; P504S） ,P63 gene, Keratin 5/6（CK5/6） and 34βE12 （K903） in prostate biopsy specimens from benign and malignant prostate diseases. Methods A total of 50 prostate needle biopsy specimens,including 30 cases of prostate cancer and 20 cases of benign prostatic hyperplasia, from January 2008 to Department 2008, were collected, and immunohistochemieal staining（ S-P method） was carried out for the expression of P504S,P63 ,CK5/6,34βE12. Results P504S expression was positive in biopsy specimens of prostate cancer, but negative in malignant prostate diseases（ P 〈 0.05 ） ;while P63, CK5/ 6,34βE12 expression was positive in the basal cells in patients with malignant prostate diseases, but negative in the basal cells in patients with prostate cancer（ P 〈 O. 05 ）. Conclusion The diagnostic and differential diagnostic utility of P504S, P63 gene, CK5/6 and K903 in prostate biopsy specimens is relevant.
出处
《中华全科医学》
2014年第1期55-55,165,共2页
Chinese Journal of General Practice
