摘要
目的了解各种血清学方法检测献血者抗-HIV的效果,探讨血清学方法的恰当及妥善应用以提高抗-HIV的准确性和检出率。方法收集广西血液中心2006年1月-2012年5月2次ELISA检测抗-HIV阳性及可疑标本972例检测结果,比较2个时间段检测结果符合性;在2次检测的基础上,再增加1次高特异性ELISA、或Determine、或明胶颗粒凝集试验（PA）、或胶体金标记法检测,以蛋白免疫印迹法（WB）做确证试验,对比与统计分析检测结果。结果献血者血液标本抗-HIV ELISA 2次检测结果的符合率为86.83%（844/972）,后阶段的检测符合率91.85%（383/417）优于前阶段83.06%（461/555）（P〈0.01）,双试剂检测符合性优于单试剂检测（P〈0.01）;2次抗-HIV ELISA检测均为阳性的标本,再使用Determine、或胶体金、或ELISA、或PA试剂检测1次并阳性,其与WB确证试验阳性的符合率可分别达100%、94.96%、77.05%和58.33%;ELISA检测抗-HIV阳性的标本相应的S/CO〉10者占87.90%（138/157）;抗-HIV阳性者确证条带gp160和gp120的阳性率为100%,gp41和p24的阳性率均为96.86%（154/159）,p55和p39阳性率为62.89%（100/159）和54.09（86/159）。结论在常规应用ELISA法检测献血者抗-HIV的基础上,恰当使用其他血清学方法可提高抗-HIV的准确性和检出率。
Objective To study the effects of all kinds of serology for detection of anti-HIV in blood donors,and to explore the appropriate combination of serological methods to improve the accuracy and the detection rate of anti-HIV. Methods The specimensfrom Guangxi blood center during January 2006 to May 2013,whose results of twice anti-HIV tests via ELISA were positive were collected,and 972 cases of suspicious resultswere also collected,and the the compliances of results during two periodswere compared. On the basis of the 2 tests,we added once high specificity ELISA,or Determine,gelatin particle agglutination test（ PA）,or colloidal gold-labeled assay andused WB as a confirmatory test,and then all results were compared and statistically analyzed. Results The coincidence rate between the first and the second anti-HIV ELISA detection results was 87. 14%（ 847 /972）,but the second was 91. 85%（ 383 /417） better than the first 83. 06%（ 461 /555）（ P 0. 01）,and double reagent for detection was better than the single reagent（ P 0. 01）. For the specimens whose results of twice anti-HIV ELISA detection were all positive,we added once Determine,or colloidal gold-labeled assay,or ELISA,or PA and their results were still positive. Theirresults and WB confirmatory tests ＇ positive coincidence rate can be amounted to 100%,94. 96%,77. 05% and 58. 33% respectively. Thesamples whose value of S / CO was greater than 10 in anti-HIV positiveaccounted for 87. 90%（ 138 /157）. In anti-HIV positive,the positive rate ofbands gp160 and gp120 was 100%,both gp41 and p24 were 96. 86%（ 154 /159）,p55 and p39 was lower and just 62. 89%（ 100 /159） and54. 09（ 86 /159） respectively. Conclusion Based on the conventional ELISA method in detection of anti-HIV in blood donors,other serological methods can improve the accuracy and the detection rate of anti HIV.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2013年第12期1218-1221,共4页
Chinese Journal of Blood Transfusion
基金
广西壮族自治区卫生厅立项课题(Z2011312)
