摘要
将含有cfp(cyan fluorescent protein)基因质粒的荧光标记根瘤菌S.LZgn5-cfp6、S.LZgn5-cfp16、S.LZgn5-cfp28,S.12531-cfp2、S.12531-cfp13、S.12531-cfp26、S.LH3436-cfp1、S.LH3436-cfp2、S.LH3436-cfp6接种于甘农5号紫花苜蓿,测定苜蓿幼苗生物量、单株结瘤数、固氮酶活性等,结果表明:荧光标记根瘤菌可有效促进植株生长和生物量的积累,有明显促生效果;与出发菌相比,荧光标记根瘤菌未对苜蓿幼苗产生显著的不良影响,能在植物体内稳定表达,具有与出发菌株相近的促生能力。
Fluorescence marked rhizobias （S. LZgnS-cfp6, S. LZgnS-cfp16, S. LZgn5-cfp28, S. 12531-cfp2, S. 12531-cfp13, S. 12531-cfp26, S. LH3436-cfpl, S. LH3436-cfp2, S. LH3436-cfp6） that contained the plasmid with cfp （cyan fluorescent protein） gene were inoculated to Gannong No. 5 alfalfa. Biomass,nodule number, nitroge- nase activity and so on were measured. The result showed that the fluorescence marked rhizobias could promote plant growth and biomass accumulation of inoculated alfalfa. Compared with their corresponding original rhizo- bias, the fluorescence marked rhizobias did no harm to alfalfa seedling, and could express stably in alfalfa plant, and had the similar growth promotion ability with original rhizobias.
出处
《草原与草坪》
CAS
2013年第6期1-8,共8页
Grassland and Turf
基金
国家牧草产业技术体系(ARS-35)资助
关键词
根瘤菌
接种
苜蓿幼苗
生物量
固氮酶活性
rhizobia ；inoculation ；alfalfa plant ；biomass ；nitrogenase activity
