摘要
目的:建立瓜馥木中总生物碱含量测定的方法。方法:用分光光度法测定瓜馥木中总生物碱的含量。以瓜馥木碱甲为对照品,溴甲酚绿为酸性染料,缓冲溶液pH值=4.8条件下,用8 mL氯仿萃取3次,于407 nm波长处测定其吸光度。结果:瓜馥木碱甲在3.16-18.96μg/L浓度范围内线性关系良好,回归方程A=0.0701C+0.0041,r=0.9989,精密度良好,RSD%=1.34%(n=5);样品溶液显色3.0 h内基本稳定,吸光度的RSD%=1.07%;本法测定的重复性良好,RSD%=1.34%;平均加样回收率为100.87%,RSD%=2.25%。结论:该法简便、准确,可用于瓜馥木中总生物碱的含量测定。
Objective： To set up a method for quantitatively determination of total alkaloids in Oldham Fissistigma Root. Methods： The content of total alkaloids in Oldham Fissistigma Root was determined by spectrophotometry. Fissistigine A was taken as a reference substance, and bromocresol green as acidic dye. The absorbance was determined when pH value of buffer solution was 4.8; And 8 mL chloroform extractive times were three and determining wavelength was 407 nm. Results： Fissistigine A showed a good linear relationship at range of 3.16 I^g /L to 18.96 μg/L; the regression equation showed A = 0.0701C＋0.0041（r=0.9989） and precision was good （RSD%=1.34%, n=5）. The sample solution was stable with in 3 hours after coloration （RSD%=1.07%）. The method had a higher repeatability （RSD%=1.34%）. The average recovery was 100.87% （RSD% =2.25%）. Conclusion： The method is easy and accurate, which can be used in the quantitative determination of total alkaloids in Oldham Fissistigma Root.
出处
《中医药导报》
2014年第1期86-88,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
湖南省大学生研究性学习和创新性实验计划项目(2012年)
关键词
分光光度法
瓜馥木
酸性染料
生物碱
瓜馥木碱甲
含量测定
Spectrophotometry
Oldham Fissistigma Root
Acidic dye
Alkaloids
Fissistigine A
Quantitative determination
