摘要
对诱变获得展青霉LD100-1菌株进行固态培养工艺优化,为提高灰黄霉素产量提供基础数据。结果表明,LD100-1最佳固态培养参数为以晚籼米、NaNO3作为碳源和氮源,初始料水比=1∶0.75,添加1.1%NaCl作为Cl-来源,再添加适量疏松剂。以培养44h的种子液接种,接种量为10%。培养过程采用变温发酵1d^7d、28℃,8d^14d、25℃。工艺优化后灰黄霉素效价达160000μg/g,提高了60%。
Solid cultivation process optimization of Penicillium patulum LD 100-1 which has been induced mutation to supply basic data for approving griseofulvin production.The results showed that the optimum conditions were as follows：indica rice and NaNO3 as carbon and nitrogen sources,the initial ratio of material to water 10.75,addition of 1.1％ NaCl as Cl-sources with bulking agent.Vaccinating 10％ of the seed liquids which have been fostered for 44h; using variable temperature fermentation of 1d～7d 28℃ and 8d～14 d 25℃.The griseofulvin titer has increased by 60000μg/g（60％） after optimization.
出处
《中国酿造》
CAS
2013年第12期57-60,共4页
China Brewing
基金
福建省“大学生创新创业训练计划”项目(SJCXCY-2012024)
关键词
展青霉
固体发酵
灰黄霉素
工艺优化
Penicillium patulum
solid fermentation
griseofulvin
technology optimazation
