摘要
从石油污染土壤中分离、筛选出4株产脂肪酶高的微生物菌株,采用正交试验对4株菌产脂肪酶发酵条件进行了初步优化。结果表明,碳源种类和油含量对各种菌株产酶的影响效果较大;其中D4菌株产酶活最高,达到51.667U/mL;而A6菌株产酶活最低,为23.334U/mL。为进一步工业化生产脂肪酶奠定理论基础。
Four high lipase-producing microbes were separated and screened from petroleum contaminated soil.Orthogonal experiment was used for four strains lipase fermentation conditions optimization.The results showed that various carbon species and oil content affected greatly to the enzyme production; the D4 strain had the highest activity which can reach 51.667U/ml.However the A6 strain producing activity was the lowest with 23.334U/ml.The experiment can be used for further theoretical foundation of lipase industrial production.
出处
《中国酿造》
CAS
2013年第12期70-73,共4页
China Brewing
基金
甘肃省自然科学研究基金计划项目(1308RJZA187)
西北民族大学中央高校基本科研业务费专项基金项目(2014106)
关键词
微生物菌株
发酵
脂肪酶
条件优化
microbial strains
fermentation
lipase
conditions optimization
