摘要
目的人工合成紫铆素(butin)的完全抗原并进行鉴定。方法采用氯乙酸钠(SMCA)与紫铆素中的羟基反应形成羧甲基醚衍生物(紫铆素-SMCA),再通过羧基与载体蛋白(BSA、OVA)偶联形成完全抗原,以TLC法鉴别是否偶联产生新的大分子化合物,并以紫外扫描分析完全抗原的偶联比率,将得到的免疫原紫铆素-BSA按免疫程序免疫Balb/c小鼠。结果 TLC显示已经形成新的大分子化合物;紫外扫描法测定紫铆素-BSA的偶联比为6∶1,紫铆素-OVA的偶联比为5∶1。采用间接酶联免疫测定,产生的抗血清的稀释度可以达到1∶4 000。结论经过TLC法、紫外扫描法和免疫方法鉴定,成功合成了紫铆素人工抗原。
Objective To synthesize artificially and identify the complete antigen ofbutin. Methods Butin was reacted with sodium chloroacetate （SMCA） to form butin-SMCA, and then coupled respectively to bovine serum albumin （BSA） and ovalbumin （OVA） to form complete antigen. The TLC method was used for the identification of new large molecular compound. The coupling ratio was determined by ultraviolet spectrometry and the antigenicity was examined using animal immune method. Results New macromolecular compound was detected by TCL method. The coupling ratio ofbutin-BSA was 6 ： 1 and that ofbutin-OVA was 5 ： 1. The indirect enzyme liked immuno sorbent assay （ELISA） showed that the butin artificial antigen had the production of specific antibody with the titer as 1 ： 4 000. Conclusion This method could synthesize butin artificial antigen successfully and produce the antibody to butin in the serum of immunized mice.
出处
《中草药》
CAS
CSCD
北大核心
2014年第3期337-340,共4页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81373542
81274043)
新疆维吾尔自治区自然科学基金资助项目(201318101)
关键词
紫铆素
人工抗原
氯乙酸钠
酶联免疫分析法
TLC
butin
artificial antigen
sodium chloroacetate
enzyme liked immuno sorbent assay
TLC
