摘要
使用一定浓度的印楝提取物 (NeemEC)处理的构树枝条作为天牛的食物 ,研究了其对桑天牛产卵量及卵孵化率的影响。在室温下 ,将成对的天牛放在盖有铁砂网的直径 2 5cm高的塑料桶中进行实验。用含印楝素 50× 1 0 -6g/mL的印楝提取物处理枝条 ,处理组天牛 ( 7对 )在 1 5d试验中 ,其每天对平均产卵量为2 0 0± 1 56个 ,而对照组每天平均产卵量为 1 0 33± 3 33个。经T—检验处理组与对照组之间差异显著。用含印楝素 1 0 0× 1 0 -6g/mL的印楝提取物处理枝条 ,在 1 5d试验中 ,处理组 7对天牛从第 5d开始死亡 ,至第 8d全部死亡 ,按实际成活天数计算 ,其每天每对平均产卵量为 1 0 0± 1 4 1个 ,而对照每天每对平均产卵量为 1 3 73± 2 90个。经T—检验两者之间差异显著。取食含印楝素 50× 1 0 -6g/mL的印楝提取物处理枝条后天牛产的卵需经 1 9d达到其最终孵化率 ,33 33% ;而对照组 9d达到最终孵化率 98 96%。在进行了 2 4d的选择取食试验中含印楝素 1 0 0× 1 0 -6g/mL的印楝提取物处理枝条 ,1 0对对照组天牛每天每对平均取食量为 3 53± 0 38g,而处理组天牛每天每对平均取食量 3 4 2± 0 60 g。经T—检验两者差异不显著。这说明桑天牛不能分辩含印楝提取物的食物 ,即印楝提取物对桑天牛不具拒食性。?
The branches of \%Broussonetia papyrifera\% Linn. were treated with neem extracts(Neem EC) as the food of the beetle (\%Apriona germari\% Hope), the effects of neem extracts on the oviposition and egg hatch of the beetle were investigated. The testing pair beetles were put into the plastic containers with a diameter of 25 cm and of a height 25 cm and covered with iron screen. During 15 days testing period, the average egg number laid per pair beetles per day in treated group (7 pairs), which were fed on branches treated by neem extracts containing 50×10 -6 g/mL azadiractin was 2.00±1.56 and that in the control group (7 pairs) was 10.33±3.33. T\|test showed significant difference in the values of the average egg number laid between treated and control groups. In the test with a dosage of the neem extracts containing 100×10 -6 g/mL azadirachtin, in the treated group (7 pairs), the beetles began to die on the fifth day and all the beetles were died on the eighth day, their average egg number laid per pair for the living beetle pairs per day, was 1.00±1.41 and that for the control group was 13.73±2.90. T\|test showed the difference between the two values was significant. The hatching rate of the eggs laid by the beetles, which had fed on the treated branches with a dosage of neem extracts containing 50×10 -6 g/mL azadirachtin, reached 33.3% at the nineteenth day. However, that of eggs laid by the control beetles was 98.9% on the ninth day. During a 24 days\' choice test of feeding, the branches were treated with a dosage of the neem extracts containing 100×10 -6 g/mL azadirachtin and the average food taken by the beetles in the treated group (7 pairs) was 3.53±0.38g per pair per day and that on the control group(7 pairs) was 3.42±0.60g per pair per day. T\|test showed that the difference between the two values was insignificant. It indicated that the beetle could not distinguish the food treated with neem extracts, that is, neem extracts is not an antifeedant for the beetle. Based on the above results a new way using neem extracts to control the beetle was put forward and discussed.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2001年第1期96-100,共5页
Scientia Silvae Sinicae
基金
国家自然科学基金(No.39870623)资助项目
关键词
印楝提取物
桑天牛
产卵
孵化率
构树
Neem extracts
Apriona germari Hope
Oviposition
Egg hatching rate
Broussonetia papyrifera Linn
