摘要
背景:运动促进冠状动脉侧支循环生成涉及众多的促血管生成相关因子,单纯对一个因子的研究很难明确侧支循环生成的信号通路和传导途径,许多促血管生长因子都与激肽释放酶-激肽系统相关,而运动对该系统的影响目前未见报道。目的:观察运动诱导对心肌梗死大鼠缓激肽表达的影响。方法:健康Wistar大鼠30只,随机分为对照组、心肌梗死组及运动组。对照组只开胸,缝扎点穿线,不进行冠状动脉结扎;其余2组制备心肌梗死模型。运动组在成功造模后给予跑台运动,30 min/d,运动4周。实验终点时取血以ELISA法检测大鼠血清缓激肽水平,采用左心房注射微球法取大鼠心肌组织测定相对血流量。结果与结论:实验结束时运动组缓激肽水平显著高于心肌梗死组(P<0.001),心肌梗死组缓激肽水平显著高于对照组(P<0.05)。心肌相对血流量实验结束时心肌梗死组、运动组均显著高于同组实验开始前(P<0.05,P<0.001),实验结束时运动组心肌相对血流量显著高于心肌梗死组(P<0.01)。各组大鼠血清缓激肽含量与心肌相对血流量存在显著相关性。提示运动可以刺激缓激肽表达水平显著升高,使心肌血流量明显增加,说明激肽释放酶激肽系统在运动诱导的血管新生中发挥作用。
BACKGROUND： Exercise can elicit generation of coronary collateral circulation through numerous pro-angiogenic growth factors. It is not enough via a single factor to clearly definite signaling pathways for the generation of collateral circulation. Many pro-angiogenic growth factors are involved in kallikrein-kinin system, and there is no report about exercise effects on this system.
OBJECTIVE： To study effects of exercise training on bradykinin expression in rats with myocardial infarction.
METHODS： Thirty Wistar rats were randomly assigned to control group, myocardial infarction group and exercises training group. Rats in the control group were subjected to thoracotomy with no coronary artery ligation. Rats in the other two groups were modeled. In the exercise training group, 3 days after myocardial infarction, rats were subjected to 30-minute exercise training on treadmill, once a day for 4 weeks. At the terminal of the experiment, blood samples were obtained to analyze bradykinin expression by enzyme linked immunosorbent assay, and the myocardium was sampled to analyze relative blood flow by microsphere method.
RESULTS AND CONCLUSION： After exercise training, bradykinin level in the exercise training group was significantly higher than that in the myocardial infarction group （P 〈 0.001）. At the end of experiment, the relative blood flows of the exercise training and myocardial infarction groups were both increased compared with before experiment （P 〈 0.05, P 〈 0.001）. At the end of experiment, the relative blood flow of the exercise training group was significantly higher than that of the myocardial infarction group （P 〈 0.01）. In all the three groups, bradykinin level was significantly correlated with the relative blood flow in the myocardium. These findings indicate exercises training can improve bradykinin expression apparently and increase blood flow in the myocardium, which illustrates that the kallikrein-kinin system plays an important role in exercise induced angiogenesis.
出处
《中国组织工程研究》
CAS
CSCD
2014年第2期239-244,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助项目(81071598)
大连市科技局项目(2009E12SF142)~~
