摘要
目的 应用牙周膜细胞（periodontal ligament cells,PDLC）膜片、骨髓基质细胞（bone marrow stromal cells,BMSC）膜片与自体牙根体外构建牙生物性种植体,研究构建具有新生牙周组织支持的生物性牙根的可行性.方法 拔除2只比格犬下颌两侧双尖牙制作缺牙区,将比格犬自体牙根预备成近似圆柱状.培养犬第3代PDLC和BMSC成细胞膜片,体外将PDLC和BMSC膜片单独或共同包裹于牙根表面构建自体牙根种植体,将种植体分为4组：①双膜片组（每只犬2颗）,将PDLC、BMSC先后包裹于同一牙根表面;②PDLC膜片组（每只犬2颗）,将PDLC单膜片包裹于牙根表面;③BMSC膜片组（每只犬2颗）,将BMSC单膜片包裹于牙根表面;④无膜片组（空白对照组）（每只犬1颗）,无膜片牙根.制作缺牙区手术8周后,将种植体植入自体缺牙区牙槽嵴,12周时取材制作石蜡切片行HE和Masson三色染色,观察各组切片的组织学结果.结果 PDLC膜片组种植体根面可见包括牙骨质、牙周膜及牙槽骨的牙周组织样结构,其中可见类似牙周穿通纤维样组织垂直插入根面及骨面;双膜片组种植体和BMSC膜片组种植体根面多为骨性结合;空白对照组亦未见牙周组织样结构形成.结论 PDLC膜片能促进牙生物性种植体的牙周再生,可部分再生出包括牙骨质、牙周膜和牙槽骨的复杂牙周组织.
Objective To fabricate the bio-implant supported by regenerated periodontal tissue utilizing periodontal ligament cells（PDLC）-bone marrow stromal cells（BMSC） sheet and natural root. Methods Premolars of 2 beagle dogs were extracted to prepare the implanted area. Auto）ogous tooth roots were carved into cylinders. PDLC and BMSC separated from beagle dogs were cultured into cell sheets in medium. Tooth roots were wrapped by one type of cell sheets or both to fabricate bio-implant and divided into four groups, tooth roots were wrapped by PDLC sheets and BMSC sheets successively（2 samples each dog） ,tooth roots were wrapped by PDLC sheets alone（2 samples each dog）, tooth roots were wrapped by BMSC sheet alone（2 samples each dog）, tooth roots without ceil sheet （1 sample each dog）. The implants were implanted into the mandibles. The mandibles were dissected 12 weeks later, sliced and stained by HE and Masson dyes for histological examination. Results In the PDLC cell sheet/root implants, histological examination revealed that new periodontal-like tissue including cementum, periodontium and alveolar bone was regenerated. In the BMSC implants,tooth ankylosis was observed. Conclusions PDLC sheet and natural root can be used to fabricate bio-implant. PDLC sheet could promote periodontal regeneration.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2014年第2期84-88,共5页
Chinese Journal of Stomatology
基金
国家自然科学基金（30772418、81271152）
上海市科学技术委员会科研计划（074119514）
关键词
牙种植体
组织工程
再生
牙周膜细胞
Dental implants
Tissue engineering
Regeneration
Periodontal ligament cell
