摘要
目的 观察黄芩、白芍水提取物对牙周炎小鼠血清IgG1和IgG2a的影响,比较黄芩、白芍对小鼠牙周炎的治疗效果,探讨黄芩和白芍对牙周炎的免疫干预和治疗机制.方法 将64只清洁级12周龄雄性昆明小鼠按随机数字表法分为4组,每组16只：①正常对照组;②牙周炎组,使用丝线结扎上颌第一磨牙和结扎部位涂菌的方法4周建立小鼠实验性牙周炎模型,于建模后第5周开始用蒸馏水灌胃;③黄芩治疗组,同法复制牙周炎模型,从建模后第5周开始用黄芩混悬液灌胃;④白芍治疗组,同法复制牙周炎模型,从建模后第5周开始用白芍混悬液灌胃.各组动物分别于建模后第4、6、8和10周末处死,每个时间点分别处死4只.制做牙体牙周组织联合切片,显微镜下观察牙周组织的病理变化.酶联免疫吸附测定（enzyme-linked immunosorbent assay,ELISA）法检测血清中抗体IgG1和IgG2a的水平.结果 牙周炎组可见大量炎性细胞浸润,牙槽骨进行性吸收,可见大量破骨细胞;黄芩治疗组和白芍治疗组牙周组织炎症程度较牙周炎组明显减轻,修复反应明显.IgG2a抗体水平：黄芩治疗组（6周：0.934±0.006,8周：0.743±0.009,10周：0.674±0.008）和白芍治疗组（6周：1.023±0.032,8周：0.851±0.032,10周：0.790±0.009）在同组各时间点之间比较差异均有统计学意义（P＜0.01）;与白芍治疗组在同时间点相比,黄芩治疗组血清IgG2a下降明显（P＜0.01）.IgG1抗体水平：黄芩治疗组（6周：0.314±0.006,8周：0.344±0.004,10周：0.367±0.006）和白芍治疗组（6周：0.287±0.005,8周：0.303±0.058,10周：0.336±0.006）在同组各时间点之间比较均随时间显著升高,黄芩治疗组较白芍治疗组血清IgG1水平在同时间点均显著升高（P＜0.01）.结论 黄芩、白芍水提取物能抑制小鼠实验性牙周炎的Th1细胞反应,增强Th2细胞反应,对小鼠实验性牙周炎有明显的治疗效果;黄芩对小鼠牙周炎的调节效果优于白芍.
Objective To examine the effect of aqueous extracts of Scutellaria baicalensis Georgi and Radix paeoniae Alba on periodontitis mice and compare the results of the two herbs for the treatment of the periodontitis mice. Methods Sixty-four SPF 12-week-old male Kunming mice were selected and randomly divided into four groups ： Control group （ C ） ; Experimental periodontitis group （P） ： the peridontitis models in Kunming mice were prepared by wrapping silk ligature and inoculating with putative periodontopathic bacteria; Scutellaria baicalensis Georgi treatment group（SG） ： periodontitis was induced by the same method described above, the mice were gavaged with Scutellaria baicalensis Georgi; Radix paeoniae Alba treatment group （RG） ： periodontitis was induced by the same method described above, the mice were garaged with Radix paeoniae Alba. Four mice were sacrificed at each time point of the end of 4,6, 8 and 10 weeks in each group. The histopathological changes of periodontal tissue were observed under microscope with HE staining. The level of serum IgG1 and IgG2a was measured by enzyme-linked immunosorbent assay（ELISA）. Results A serious inflammatory response, alveolar progressive absorption and a large number of osteoclasts were observed in the experimental periodontitis group. However, in SG and RG,the inflammation of the periodontal tissue was decreased and tissue repair was significant. The level of serum IgG2a in SG（ 6 week ： 0. 934 ± 0. 006,8 week ： 0. 743 ± 0. 009,10 week ： 0. 674 ± 0. 008 ） and RG （ 6 week ： 1. 023 ± 0. 032,8 week ：0. 851 ± 0. 032,10 week ：0. 790 ± 0. 009 ） was significantly decreased after the mice were gavaged with the two herbs（ P 〈 0. 01 ）. The level of serum IgG2a in SG was signihcantly lower than that of RG（P 〈0.01）. The level of serum IgG1 in SG（6 week：0.314 ±0.006,8 week：0.344 ± 0. 004,10 week ： 0. 367 ± 0. 006 ） and RG （ 6 week ： 0. 287 ± 0. 005,8 week ： 0. 303 ± 0. 058, 10 week ： 0. 336 ±0. 006） were significantly increased（ P 〈 0. 01 ）. The level of serum IgG1 in SG was significantly higher than that of RG （ P 〈 0. 01 ）. Conclusions Both the aqueous extracts of Scutellaria baicalensis Georgi and Radix paeoniae Alba showed therapeutic effect on periodontitis in mice. Scutellaria baicalensis Georgi was more effective than Radix paeoniae Alba.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2014年第2期89-94,共6页
Chinese Journal of Stomatology
基金
广东省自然科学基金（S2013010016736）
关键词
牙周炎
黄芩
Th1-Th2平衡
白芍
Periodontitis
Scutellaria baicalensis
Th1-Th2 balance
Radix paeoniae Alba
