摘要
目的探讨缩宫素(OT)对大鼠急性心肌缺血再灌注后一氧化氮(NO)与内皮素-1(ET-1)的影响及可能的机制。方法将大鼠随机分为心肌缺血再灌注组(I/R组)、I/R+OT预处理组(I/R+OT组)、假手术组(SH组)和健康对照组(N组)。采用在体大鼠结扎冠状动脉前降支25min后,松扎再灌注120min形成I/R模型,SH组只穿线不结扎。I/R+OT组在缺血前25min腹腔注射OT 0.03μg/kg,其余3组给予等量生理盐水。再灌注结束后测定血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、NO及ET-1;再灌注结束时,采用HE染色进行心肌组织形态学分析。结果光镜下观察,N组与SH组心肌细胞排列整齐,无肌纤维破坏,I/R组心肌纤维排列不规则、断裂,心肌间质水肿,较多粒细胞灶状浸润,I/R+OT组可见心肌细胞排列、间质水肿及粒细胞浸润程度介于N组与SH组。与SH组相比,I/R组和I/R+OT组血清CK-MB、LDH、ET-1升高,NO水平降低,差异均有统计学意义(P均<0.05),而N组的表达差异无统计学意义(P>0.05);与I/R组相比,I/R+OT组血清CK-MB、LDH、ET-1降低,NO水平升高,差异均有统计学意义(P均<0.05)。I/R组和I/R+OT组血清NO与ET-1水平呈负相关(P<0.05)。结论 OT预处理对大鼠I/R有保护作用,其作用机制可能与OT增加血清NO水平、降低ET-1水平有关。
Objective To investigate the effect and possible mechanism of oxytocin on the expression of NO and ET-1 in precon-ditioning myocardium of rats with ischemia .Methods The rats were randomly divided into ischemic reperfusion group（I/R） ,ische-mic reperfusion ＋ oxytocin pretreatment group（I/R＋OT） ,sham operation group（SH） and nomal control group（N） .The myocar-dial ischemia-reperfusion model was induced by 25 min coronary occlusion and 120 min reperfusion in opened-chest anesthetized rats ,rats of SH group were not conducted with ligation but threading only .Group I/R＋ OT were administered oxytocin（0 .03 g/kg ,i .p）25 min prior to ischemia ,the other three groups were given the same amount normal saline .After reperfusion ,nitric oxide （NO） ,endothelin-1（ET-1） ,creatin kinase isoenzyme（CK-MB）and lactate dehydrogenase（LDH）in blood serum were measured .At the end of reperfusion ,HE stain was used to observe the morphology of the cardiac muscular tissue .Results Pathological observa-tion showed that the myocardial cell in group SH and in X ranged in order ,with no fibers fractured ,myocardial fibers in I/R group were fractured and disorganized ,interstitial edema ,neutrophil infiltration were also observed .It was more serious in group I/R than in group I/R＋OT .Compared with SH group ,the CK-MB ,LDH ,ET-1 of the I/R group and I/R＋OT group increased ,the NO de-creased（P〈0 .05） ,and it had no difference in group N and group SH （〉0 .05） Compared with the I/R group ,in I/R＋OT group the expression of CK-MB ,LDH ,ET-1 were decreased ,the level of NO increased（P〈0 .05） .There was a negative correlation be-tween NO and ET-1 in group I/R and I/R＋ OT （P〈 0 .05） .Conclusion Oxytocin pretreatment on rat myocardial ischemia and reperfusion has protective effect ,and its action mechanisms maybe associated with the increase of the content of NO and the de-crease of the content of ET-1 in blood serum .
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第4期471-473,共3页
Chongqing medicine
