摘要
为研究黄芩苷能否增强喹诺酮类药物环丙沙星对鸵鸟源大肠杆菌的抗菌活性，本试验先用二倍稀释法测定单个药物对鸵鸟源大肠杆菌的抗菌活性，进而筛选出10株耐药菌株（T1～T4、T7、T8、T10、T12、T21、T25），然后采用微量肉汤稀释棋盘法，用环丙沙星和黄芩苷对以上10株耐药菌株进行体外联合药敏试验。结果发现环丙沙星和黄芩苷单用的MIC分别为64和512或1024 μg/mL；联合用药后，对10株耐药菌的分级抑菌浓度进行测定，其中T1、T2、T3、T7、T8、T10、T12、T21的分级抑菌浓度均为0.375，T4、T25的分级抑菌浓度均为0.25，10株菌的分级抑菌浓度均小于0.5。结果表明，环丙沙星和黄芩苷联合对鸵鸟源大肠杆菌起协同作用。
The assay was to study the sensitivity of the pathogenic Escherichia coli from ostrich to ciprofloxacin combined with baicalin. First, ciprofloxacin and baicalin against Escherichia coli from ostrich were detected by double dilution method, the resistant Escherichia coli from ostrich were T1 to T4, T7,T8,T10,T12,T21,T25. Then Escherichia coli from ostrich were induced broth dilution checker-board method, when ciprofloxacin and baicalin were used alone, the MIC were 64, 512 or 1024 μg/mL,respectively, when the use of ciprofloxacin and baicalin were combined, the fractional inhibitory concentrations （FIC） of Escherichia coli from ostrich （T1，T2，T3，T7，T8，T10，T12 and T21） were all 0.375, the FICs of Escherichia coli from ostrich （T4，T25） were both 0.25, all were below 0.5. The results suggested that the combination of ciprofloxacin and baicalin demonstrated synergistic effect on the Escherichia coli from ostrich.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第1期214-217,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(U1304328)
河南省科技厅项目(132300410113)
河南农业大学博士启动基金(30200444)
关键词
环丙沙星
黄芩苷
鸵鸟源大肠杆菌
联合药敏试验
分级抑菌浓度
ciprofloxacin
baicalin
Escherichia coli from ostrich
combined susceptibility test
fractional inhibitory con-centration （FIC）
