摘要
目的:观察中药健脾解毒方对人结肠癌HCT116/奥沙利铂(oxaliplatin,L-OHP)裸鼠皮下移植瘤化疗的增效作用,初步探讨其增效的作用机制.方法:建立人结肠癌耐奥沙利铂细胞株HCT116/L-OHP裸鼠皮下移植瘤模型,随机分为空白组、奥沙利铂组、健脾解毒方组、健脾解毒方低剂量联合奥沙利铂组、健脾解毒方高剂量联合奥沙利铂组.治疗中测量肿瘤的长径和短径,根据公式计算瘤体体积,描绘瘤体生长曲线,治疗结束后称取瘤质量,公式计算瘤体抑制率和瘤质量抑制率;免疫组织化学,Western blot技术检测皮下移植瘤Caspase3的表达.结果:健脾解毒方高剂量联合奥沙利铂组的瘤体体积和瘤质量(995.54 mm3±87.26 mm3;0.85g±0.06 g)及健脾解毒方低剂量联合奥沙利铂组的瘤体体积和瘤质量(1318.32 mm3±100.68mm3;1.06 g±0.07 g)明显低于单用健脾解毒方组(1967.83 mm3±178.83 mm3;1.71 g±0.11 g)或奥沙利铂组(1698.46 mm3±147.61 mm3;1.56g±0.12 g),有统计学意义(P<0.05或P<0.01);同时健脾解毒方高剂量联合奥沙利铂组的瘤体抑制率和瘤质量抑制率(55.63%;56.85%)及健脾解毒方低剂量联合奥沙利铂组的瘤体抑制率和瘤质量抑制率(41.25%;46.19%)明显高于单用健脾解毒方组(12.30%;13.20%)或奥沙利铂组(24.31%;20.81%),有统计学意义(P<0.05或P<0.01);健脾解毒方联合奥沙利铂组均可上调裸鼠皮下移植瘤组织Caspase3蛋白的表达有统计学意义(P<0.05或P<0.01).结论:健脾解毒方联合奥沙利铂能抑制裸鼠结肠癌皮下移植瘤的生长,增加奥沙利铂的疗效,其增效的机制是通过上调Caspase3的表达而促进细胞凋亡有关.
AIM： To observe whether Jianpi Jiedu decoction and oxaliplatin （L-OHP） have a synergistic effect on human colon cancer HCT116/L-OHP xeno- grafts in nude mice and to explore the underly- ing mechanism. METHODS： A xenograft tumor model was es- tablished using L-OHP-resistant human colon cancer HCT116 cells in nude mice. Mice were randomly divided into a control group, a L-OHP group, a Jianpi Jiedu decoction group, a low dose Jianpi Jiedu decoction plus L-OHP group, and a high dose Jianpi Jiedu decoction plus L-OHP group. During the treatment process, the long and short diameters of tumors were mea- sured to calculate tumor volume according to the formula and depict tumor growth curve. After treatment, the tumor weight was measured to calculate tumor inhibition rate and tumor weight inhibition rate. The expression level of Caspase 3 in tumor xenografts was detected us- ing immunohistochemistry and Western blot. RESULTS： Tumor volume and tumor weight were significantly lower in the high dose Jianpi Jiedu decoction plus L-OHP group （995.54 mm3±87.26 mm3; 0.85 g ±0.06 g） and low dose Jianpi Jiedu decoction plus L-OHP group （1318.32 mm3 ± 100.68 mm3; 1.06 g ± 0.07 g） than in the Jianpi Jiedu decoction group （1967.83 mm3 ± 178.83 mm3; 1.71 g ± 0.11 g） and L-OHP group （1698.46 mm3 ± 147.61 mm3; 1.56 g ± 0.12 g） （P 〈 0.05 or P 〈 0.01）. Tumor inhibition rate and tumor weight inhibition rate were significantly higher in the high dose Jianpi Jiedu decoction plus L-OHP group （55.63%; 56.85%） and low dose Jianpi Jiedu decoction plus L-OHP group （41.25%; 46.19%） than in the Jianpi Jiedu decoction group （12.30%; 13.20%） and L-OHP group （24.31%; 20.81%） （P 〈 0.05 or P 〈 0.01）. Jianpi Jiedu decoction plus L-OHP significantly increased the expression of Caspase3 protein in tumor xeno- grafts in nude mice compared to other groups （P 〈 0.05 or P 〈 0.01）. CONCLUSION： Jianpi Jiedu decoction plus L-OHP can inhibit tumor growth of xenografts in nude mice and increase the efficacy of L-OHP possibly by up-regulating the expression of Cas- pase 3 to promote apoptosis.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第3期364-370,共7页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.81073105
上海市科学技术委员会基金资助项目
No.10ZR1427300~~
关键词
健脾解毒方
结肠癌
凋亡
半胱天冬酶3
Jianpi Jiedu decoction
Colon carci-noma
Apoptosis
Caspase3
