摘要
目的探讨不同营养模式对宫内发育迟缓幼鼠胰岛素样生长因子及受体表达的影响。方法用母鼠全程饥饿法建立宫内发育迟缓(IUGR)动物模型,IUGR新生雌鼠随机分为4组,新生幼鼠母乳喂养,各组分别给予母鼠下述相应饮食饲料喂养3周至断乳:①IUGR对照组给予常规饮食;②IUGR高糖饮食组(A组);③IUGR高蛋白饮食组(B组);④IUGR高脂饮食组(C组)。各组幼鼠在3周哺乳期间母鼠分别摄取上述饲料。各组幼鼠断乳后同样分别摄取上述饲料。另以正常母鼠所生正常新生雌鼠为正常对照组(C/N组)。生后母乳喂养至3周断乳,母鼠及断乳后幼鼠均给予常规饮食喂养。各组幼鼠分别取肝、肺进行免疫组织化学染色检测和胰腺组织进行苏木精-伊红染色检测。结果幼鼠多肽糖金属蛋白酶12(ADAM12)的测定结果各个月之间均无统计学意义(P>0.05)。幼鼠孕期相关血浆蛋白酶A(PAPP-A)的测定结果在各组幼鼠的第1个月有统计学意义(P<0.05),但在第2、3、4个月无统计学意义(P>0.05)。幼鼠Toll样受体(TLR-4)的测定结果各个月之间均无统计学意义(P>0.05)。幼鼠胰腺组织结果在幼鼠的第1个月有统计学意义(P<0.05),但在第2、3、4个月无统计学意义(P>0.05)。结论 ADAM12和PAPP-A的表达量或许与幼鼠的追赶生长密切相关;尽管TLR-4的检测结果在统计学上无明显意义,但高糖饮食组和高脂饮食组胰腺发生炎症的可能性较大。
Objective To investigate the effect of early different nutritional diet on the expression of IGFs and its receptor in retarded intrauterine growth rat pups. Methods The IUGR rat model was established by food restriction of pregnant rats. Newborn IUGR rats were randomly divided into four groups： IUGR model （S/N） group, IUGR high sugar diet （A） group, IUGR high protein diet （B） group, IUGR high fat （C） group. Only the mother rats were given those different diets individually, and all IUGR newborn pups were lactated for three weeks. All IUGR pups were fed with those different diets individually till the end of the month. Normal birth weight newborn rats were used as the control group fed with normal diet. Liver and lung tissues were detected by immunohistochemical staining and pancreatic tissues were stained with HE detection for all groups of rats. Results Polypeptide glycoprotein metalloprotease 12 （ADAM12） test results each month showed no statistical difference （P 〉 0.05）. The determination results of plasma protein A （PAPP-A） in rat pups was statistically different among the groups in the first month （P 〈 0.05）, but no statistical difference was found in the second month, third month, and fourth month （P 〉 0.05）. TLR-4 test results had no statistical difference （P 〉 0.05）. Rat pancreatic tissue test results were statistically different in the first month （P 〈 0.05）, but the difference was not significant in the second month, third month, and fourth month （P 〉 0.05）. Conclusion ADAM-12 and pregnancy associated PAPP-A expression results suggest that ADAM12 and PAPP-A may be closely related with rat catch-up growth, while the TLR-4 test results are not statistically different, but the chances of high glucose group and high fat group pancreatic inflammation are relatively large.
出处
《华西医学》
CAS
2014年第2期277-281,共5页
West China Medical Journal
基金
四川省科技厅基金(2012JY0007)~~
