摘要
【目的】探讨雌激素 (E2 )、孕激素 (P)及促性腺激素 (FSH)对卵巢癌细胞株体外生长的调控。【方法】采用四甲基偶氮唑蓝 (MTT)比色法比较细胞数目 ,用流式细胞仪检测细胞周期。【结果】 10 -12 mol/LE2 和 10 μg/LFSH作用 48h能显著促进卵巢癌细胞株H0 8910细胞增殖 ,E2 和FSH分别使S期和G2 /M期细胞比例增加。P能抑制卵巢癌细胞株H0 8910细胞生长 ,且对细胞生长的抑制作用对剂量有依赖性。【结论】卵巢癌细胞株H0 8910的生长受到生殖激素的调控 ,低浓度E2 和高浓度FSH具有促癌细胞增殖作用 ,孕激素则对癌细胞的生长产生明显的抑制效应。
Objective The study was carried out to investigate the effects of estradiol(E 2), follicle-stimulating hormone(FSH) and progesterone(P) on the proliferation of ovarian cancer cell line H0-8910 in vitro. Method Cultivating human ovarian serous cystadenocarcinoma cell line H0-8910 in vitro, comparing cell numbers using MTT method, flow cytometry detecting cell cycle. Results After incubation with 10 -12 mol/L E 2 or 10 μg/L FSH for 48 hours, cell proliferation increased significantly. E 2 and FSH increased the cells percentage in Sphase and G 2/M phase of cell cycle respectively P could inhibit H0-8910 cells growth significantly in a dose-dependent manner. Conclusion It is suggested that E 2 and FSH can stimulate the proliferation of ovarian cancer cell, P can inhibit cell growth.
出处
《中山医科大学学报》
CSCD
北大核心
2001年第1期29-32,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
关键词
卵巢癌
药物疗法
雌激素
孕激素
促性腺激素
药理学
ovarian neoplasm/drug therapy
estrogen/pharmacology
progesterone/pharmacology
gonadotropins/pharmacology
hormones/therapeutic use
