摘要
为了对单子叶植物重要性状相关基因启动子进行研究,以载体pGWB450为基本骨架,利用酶切方法将ubiquitin启动子序列和NPTⅡ抗性基因引入该载体,构建了新载体pUKGF。其转基因单子叶植株可通过卡那霉素进行抗性筛选;另外,该载体含有Gateway元件,启动子可高效重组到该载体中;启动子下游是绿色荧光蛋白基因,根据该报告基因的表达部位,可确定启动子功能的组织特异性。该载体在单子叶植物启动子鉴定方面将有广阔的应用前景。
In the study, the vector pGWB450 was used as a backbone,and the ubiquitin promoter and the resistance gene NPT II were integrated into the vector to construct the new vector pUKGF by enzyme digestion method,which could be screened by kanamycin in transgenic monocot plants. In addition,the new vector contained the Gateway elements which could integrate the interested promoter sequence into the vector,while the GFP gene was located in the downstream of the promoter which would confirm the regulation pattern of promoter in monocot plants. With many advantages,the pUKGF vector would have a broad application prospect in identifying the promoter function of monocot plants.
出处
《河南农业科学》
CSCD
北大核心
2014年第2期15-18,共4页
Journal of Henan Agricultural Sciences
基金
国家863计划项目(2012AA10A309)
天津市应用基础与前沿技术研究计划项目(11JCYBJC09100)
天津农学院科学研究发展基金计划(2011N11)
关键词
单子叶植物
启动子
载体构建
绿色荧光蛋白
限制性内切酶
monocotyledon
promoter
vector construction
green fluorescence protein
restriction enzyme
