摘要
采用循环伏安法和光谱法研究了羧甲基壳聚糖-Ag(CMC-Ag)配合物与鲱鱼精DNA之间的作用方式。结果发现,CMC-Ag配合物的存在导致Fe(CN)3-/4-6探针分子峰电流下降、式量电位正移,显示CMC-Ag和探针分子Fe(CN)3-/4-6与DNA之间存在竞争性作用,说明CMC-Ag通过插入方式与DNA相互作用;一定的扫描速率范围内,在CMC-Ag配合物参与的条件下,Fe(CN)3-/4-6在Au/DNA电极上的反应受吸附控制。CMC-Ag配合物使得鲱鱼精DNA的特征峰发生明显的减色效应,最大吸收峰峰位红移,进一步表明CMC-Ag配合物以插入方式与鲱鱼精DNA发生了相互作用,导致DNA分子的构象变化。
The interaction between carboxymethyl chitosan silver complex （CMC-Ag） and herring sperm DNA were investigated by cyclic voltammetry and UV-Vis spectra. The results showed that the peak current of probe molecule Fe （CN）3-/4- 6 at Au/DNA electrodes decreased obviously, and the peak potential shifted positively because of the existences of CMC-Ag. These results indicated that there was a competitive effect between CMC-Ag and probe molecule with herring sperm DNA, which revealed that binding mode between CMC-Ag and herring sperm DNA was intercalation. The reaction of Fe （CN）3-/4-6 at Au/DNA electrodes was controlled by adsorption mechanism in the range of scan rate from 0.01V/s to 0.2V/s. The absorption intensity of herring sperm DNA decreased with the adding of CMC-Ag, and the maximum absorption peak had a red shifted, which further confirmed the intercalation binding mode, and led to the change of DNA conformation.
出处
《化学通报》
CAS
CSCD
北大核心
2014年第3期285-288,共4页
Chemistry
基金
天水师范学院"青蓝"人才工程项目和天水师范学院中青年科研项目(TSA1305
TSA1307资助
