摘要
目的探讨新型梭织面料对微波辐射致大鼠脑损伤的保护作用。方法 60只Wistar雄性大鼠随机分为假辐射组、单纯辐射组、不锈钢纤维面料防护组和新型梭织面料防护组。采用30 mW/cm2微波辐射15 min,于辐射后6 h^28 d,采用Morris水迷宫检测大鼠的学习和记忆能力;高效液相色谱法(HPLC)检测大鼠海马组织中天冬氨酸(Asp)、谷氨酸(Glu)、甘氨酸(Gly)及γ-氨基丁酸(GABA)含量;光镜观察大鼠脑组织结构改变;电镜观察大鼠脑超微结构改变。结果 30 mW/cm2微波辐射后2 d、3 d和14 d,单纯辐射组大鼠学习和记忆能力下降,新型梭织面料防护组与假辐射组相比无明显差异,不锈钢纤维面料防护组与单纯辐射组相比无明显差异;30 mW/cm2微波辐射后7 d,单纯辐射组大鼠海马组织氨基酸类神经递质紊乱,新型梭织面料防护组与假辐射组相比无明显差异,不锈钢纤维面料防护组与单纯辐射组相比无明显差异;30 mW/cm2微波辐射后7 d,单纯辐射组大脑海马神经元固缩深染,血管周间隙增宽,线粒体肿胀、空化,内质网扩张,突触结构模糊,部分囊泡减少。新型梭织面料防护组大鼠脑组织损伤明显较辐射组轻,不锈钢纤维面料防护组大鼠脑组织损伤比单纯辐射组稍轻。结论 30 mW/cm2微波辐射可损伤大鼠的学习记忆能力和脑组织结构,引起大脑海马组织氨基酸类神经递质代谢紊乱;新型梭织面料对微波辐射所致的上述损伤具有良好的防护作用,其防护效果优于不锈钢纤维面料。
Objective To rats induced by microwave study irradi the protective effects of a new type woven fabric on the brain injury in ation. Methods Sixty male Wistar rats were randomly divided into control group, radiation group, stainless steel fiber group and new type woven fabric group. These rats were exposed to microwave irradiation with an average power density of 30 mW/cm2 for 15 min. From 6 h to 28 d after irradiation, the learning and memory abilities of rats were assessed by Morris water maze test. The contents of Asp, Glu, Gly and GABA in the brain hippocampus were measured by HPLC. The morphological ＇changes of brain tissues were observed by light and electron microscopy. Results The learning and memory abilities of the rats were impaired at 2 d, 3 d and 14 d after 30 mW/cm2 microwave irradiation. The changes of the new type woven fabric group were slight and similar to those in the normal group. The changes in the stainless steel fiber group were similar to those in the radiation group. Amino acids in hippocampus of rats were disturbed at 7 d after 30 mW/cm2 microwave irradiation. The changes of the new type woven fabric group were slight and similar to those in the normal group. The changes of the stainless steel fiber group were similar to those in the radiation group. The structure of brain and hippocampus were damaged at 7 d after irradiation. The neurons showed chromatin condensation, perivascular space widening, mitochondria swelling and vacuolation, endoplasmic reticulum dilatation, blurred synaptic clefts and decreased amount of synaptic vesicles. The injuries of the 30 mW/cm2 irradiation group were more serious than that of the stainless steel fiber group while the injuries of the latter group were more seri- ous than that of the new type woven fabric group. Conclusions 30 mW/cm2 microwave irradiation may impair the learning and memory abilities and brain structure in rats. Amino acids in the hippocampus are disturbed by 30 mW/cm2 microwave irradiation. The new type woven fabric may exert better protective effects on the brain injury induced by microwave radiation, and its protective effects are better than that of the stainless steel fiber fabric.
出处
《中国体视学与图像分析》
2013年第4期380-387,共8页
Chinese Journal of Stereology and Image Analysis
基金
全军后勤科研规划十二五重点项目(BSW11J008)
关键词
微波
大鼠
脑损伤
防护材料
microwave
rat
brain injury
hippocampus
protective material
woven fabric
