摘要
目的:建立雷公藤水煎剂致大鼠急性肝损伤模型。方法:分别设低、中、高给药剂量(2.5、3.75、5.0g/kg)和不同给药时间(1、2、3、4、5、6d)组,大鼠经口灌服雷公藤水煎剂,同时设正常对照组。采用称重法测定大鼠肝脏指数,干化学法检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活力,HE染色观察肝组织病理改变并进行半定量分析。结果:与正常组比较,雷公藤水煎剂中、高剂量组大鼠的肝脏指数及血清ALT、AST的活力显著升高,且肝组织均有明显病理改变,肝脏严重受损,差异均有统计学意义;随着给药时间的延长,雷公藤水煎剂的毒性呈加重趋势,干预4d后达到峰值。结论:雷公藤中剂量(3.75g/kg)干预4d可作为制备大鼠急性肝损伤模型的相对合适剂量和时间。
Objective： To establish the acute liver injury model in rats induced by Tripterygium wilfordii Hook. F （TWHF） water decoction. Methods： Rats were given TWHF water decoction by gavage and divided into different groups by doses of 2.5, 3.75, 5.0g/kg and time of 1, 2, 3, 4, 5 and 6d. At the same time, a control group was set. The liver index of rats was detected by weighing method. The activities of serum ALT and AST were detected by dry chemical method. Liver tissue pathological changes were analyzed by HE staining and semi-quantitative analysis was combined. Results： Compared with the control group, the liver index and the activities of serum ALT and AST were increased significantly in the medium and high doses of TWHF water decoction groups. The pathological changes and serious liver damage were found in liver tissue too, and the difference was statistically significant. With the extension of the intervention time, the toxic effects of TWHF had an increasing trend and arrived at the peak after 4 days. Conclusion： Rats are intervened for 4 days at the medium dose of 3.75g/kg of TWHF can be considered as relative fight methods to induce acute liver injury model.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第3期855-858,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.30873433)
山西省青年科技研究基金项目(No.2012021031-6)
山西省高校科技开发研究项目(No.20101106)~~
关键词
雷公藤
肝损伤
模型
Tripterygium wilfordii Hook. F
Liver injury
Model
