摘要
目的人类T淋巴细胞白血病病毒(HTLV-Ⅰ/Ⅱ),是一种复杂的逆转录病毒,呈世界性广泛分布,国内局部地区有相应的报道。本次调查沈阳地区无偿献血人群中人类T淋巴细胞白血病病毒感染状况,为本地区安全用血提供合适的策略。方法对沈阳地区献血人群以国产双抗原夹心法ELISA试剂初检血清中HTLV-Ⅰ/Ⅱ抗体,初检阳性标本再双孔复试,复试阳性标本采用RT-PCR或蛋白印迹法(Westemblot,WB)确证。结果从2013年3月-2013年5月止,共检测无偿献血者9 050份标本,结果检出可疑阳性5份,用RT-PCR或WB确证实验证实全部为阴性,假阳性率为0.06%。结论调查结果表明沈阳地区献血人群属于HTLV-Ⅰ/Ⅱ感染的低发区或非流行区,从血液检测成本效益和安全输血角度综合分析,沈阳地区对预防血液传染HTLV-Ⅰ/Ⅱ策略是对血制品进行滤白处理更合适。
Objective Human T lymphotropic virus （ HTLV - I / II） is a kind of complex retrovirus and widely distributed throughout world. The corresponding information was reported in local area of China. This paper aims to investigate the infection status of human T lymphotropie virus among volunteer blood donors in Sheyang, and provide appropriate strategies for blood safe- ty in this area. Methods HTLV - I / II antibody was detected in blood donors in Shenyang area by home - made double anti- gen sandwich ELISA kit in the first test. The positive blood samples in the first test were re - tested by double - hole retrial method. Then the seropositive samples were confirmed by RT - PCR or Western blot. Results A total of 9050 voluntary blood donors were detected from March to May in 2013, 5 samples were suspected positive, but confirmed negative with RT -PCR or WB, the false positive rate was 0.06%. Conclusion The blood donors belong to the low - incidence and non - epidemic area of HTLV - I / II infection in Shenyang. Based on the analysis of the blood test cost and transfusion safety, leucodepletion treatment may be better to prevent the HTLV - I / II virus infection in blood transfusion.
出处
《中国卫生检验杂志》
北大核心
2014年第4期588-590,共3页
Chinese Journal of Health Laboratory Technology
基金
国家科技重大专项(2012ZX10004702)
