摘要
目的研究口服调节性T细胞(Tregs)源性外泌体(exosomes)对大鼠移植肾存活情况的影响。方法肾移植术前5 d开始,每天给实验组大鼠用Treg细胞源性exosomes悬液灌胃(0.5 ml/天,质量浓度为1.15 g/ml),对照组以同样方式灌饲PBS溶液。术后第3、7、10及13天,颈静脉穿刺抽血,测血肌酐(Scr)和尿素氮(UN)变化。移植术后第10天,取各组大鼠移植肾做病理检测。结果实验组大鼠存活时间较空白对照组存活时间明显延长(P<0.05)。对照组大鼠Scr和UN在术后从第10天,第13天相对于实验组明显升高,而实验组则一直保持相对稳定(P<0.05)。病理结果可见实验组大鼠移植肾病理损伤情况与对照组相比明显较轻。结论灌饲Treg细胞源性exosome可延长移植肾存活时间,并可改善移植肾功能。
Regulatory T cells （Tregs） play a very important role in down-regulating immune rejection and inducing immune tolerance. Exosomes are small membrane bound vesicles secreted by most cells including Tregs. To detect whether exosomes secreted by Tregs can participate in transplantation tolerance, we fed the prepared exosomes （0.5 ml/day, 1.15 g/ml） to recipient rats in experimental group while fed PBS to those in control group the same way 5 d before renal transplantation. Peripheral blood was collected at different timepoints （3 d, 7 d, 10 d and 13 d post transplantation） for serum creatinine （Scr） and urea nitrogen （UN） measurements, and histopathology examination of allograft kidney tissue was carried out at day 10 after transplantation. We found survival time of experimental group was significant longer than that of control group （P 〈 0.05）. The levels of Scr and UN in the control group increased significantly since day 10 post-transplantation （P〈 0.05）, while those in the experimental group remained relatively stable. Histopathology examination showed transplanted kidney injury was alleviated in experimental group compared with the control group. The results suggest that Tregs-derived exosomes can prolong the survival time and improve the function of transplanted kidney.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2014年第3期197-201,共5页
Immunological Journal
基金
国家自然科学基金资助(81170693)
