摘要
目的：建立HPLC转换波长法，同时测定木瓜中绿原酸、齐墩果酸、熊果酸含量。方法：采用HPLC法，KromasoilC18（250mm×4．6mm，5μm）色谱柱，流动相：乙腈-0．6％磷酸溶液，梯度洗脱，流速：0．8mL／min，检测波长：326nm，210nm；柱温：30oC。结果：绿原酸在0．192-2．880μg（r=0．9994），齐墩果酸在0．488-7．320μg（r=0．9999），熊果酸在0．437～6．468tLg（r=1）范围内，线性关系良好；三种化合物的平均回收率为别为101．13％（RSD=1．98％，n=6）、99．64％（RSD=1．97％，n=6）及99．66％（RSD=1．90％，n=6）。结论：该方法简便准确，重现性好，可为控制木瓜质量提供参考。
Objective：To estab1ish a wave1ength conversion method of HPLC for simu1taneous determination of ch1orogenic acid,o1eano1ic acid and urso1ic acid in Fructus Chaenome1is. Methods：Stationary phase was Kro masoi1 C1s（250mm×4.6mm,5 μm） with the co1umn temperature of 30 ℃. Acetonitri1-0.6% H3PO4 was used as the mobi1e phase with gradient e1ution. The f1ow rate was 0.8 mL/min. The detection wave1ength was 326 nm and 210 nm. Resu1ts：The ch1orogenic acid had a good 1inearity re1ationship（r = 0.9994,n = 6） between 0.192N2.880 μg. The o1eano1ic acid had a 1inear range between 0.488-7.320 μg with r=0.9999（n=6）. The urso1ic acid had a 1inear range between 0.437-6.468 μg with r= 1（n=6）. The average recovery of ch1orogenic acid was 101.13% with RSD of 1.98%,the o1eano1ic acid was 99.64% with RSD of 1.97%,the urso1ic acid was 99.66% with RSD of 1.90%. Conc1usion：This method is simp1e,accurate,reproducib1e and can be used for the qua1ity contro1 of Fructus Chaenome1is.
出处
《山东中医杂志》
2014年第3期227-229,共3页
Shandong Journal of Traditional Chinese Medicine
关键词
木瓜
转换波长法
HPLC
绿原酸
齐墩果酸
熊果酸
Fructus Chaenomelis
Wavelength Conversion method
HPLC
Chlorogenic acid
Oleanolic acid
Ur-solic acid
