摘要
目的探讨转化生长因子β1（TGF-β1）基因多态性与妊娠期糖尿病（GDM）发病的相关性。方法选择80例GDM孕妇（GDM组）和80例健康孕妇（对照组），采用DNA直接测序法对TGF—B1基因的＋869T／C位点单核苷酸多态性（SNP）进行检测。结果在所有受试者中，TGF—β1基因＋869T／C位点存在TT、Tt、CC3种基因型，分布差异符合Hardy—Weinberg平衡，具有群体代表性。GDM组与对照组TGF-β1基因＋869T／C位点TT、TC、CC基因型频率及T、C等位基因频率比较差异均有统计学意义（X^2值分别为9．27、9．87，P〈0．05）。结论TGF-β1基因＋869T／C位点被证实存在多态性，TGF—β1基因＋869T／C位点SNP可能与GDM的遗传易感性相关。
Objective To investigate the correlation between transforming growth factor-J31 （TGF-[31） gene polymorphism and the pathogenesis of gestational diabetes mellitus （GDM）. Methods Eighty women with GDM （ GDM group） and 80 normal pregnant women （control group） were studied. Single nucleotide polymorphism （SNP） of TGF-β1＋ 869T/C was determined by using direct DNA sequencing. Results In all subjects there were three genotypes including TT, CT and CC. The distribution of these genotypes was found to obey Hardy-Weinberg equilibrium with representation. The differences in the frequency of TT, TC and CC at TGF-β1 ＋ 869T/C were significant, and the frequency of T and C alleles between two groups was also statistically significant （X^2 value was 9.27 and 9.87, respectively, both P 〈 0.05 ）. Conclusion TGF-β1 ＋ 869T/C polymorphisms have been confirmed. TGF-β1 ＋ 869T/C SNP may be associated with hereditary susceptibility of GDM.
出处
《中国妇幼健康研究》
2014年第1期35-37,共3页
Chinese Journal of Woman and Child Health Research
基金
温州市科技计划资助项目(Y20120111)
关键词
转化生长因子Β1
基因
单核苷酸多态
妊娠期糖尿病
相关性研究
transforming growth factor-β1 （ TGF-β1 ）
gene
single nucleotide polymorphism （ SNP ）
gestational diabetes mellitus（GDM）
correlation study
