摘要
为探讨Ca2+作为信号分子在水杨酸(SA)诱导白桦悬浮细胞三萜合成中的作用,本文用SA、Ca2+及Ca2+阻断剂对白桦悬浮细胞进行处理,采用紫外分光光度法和高效液相色谱法测定细胞中总三萜及其组分的积累量;同时利用荧光定量PCR方法检测了白桦细胞中三萜合成途径关键酶基因(FPS、SS、SE、BPW、BPY、CAS)和钙调蛋白基因表达变化。结果显示:水杨酸可诱导白桦悬浮细胞三萜物质(总三萜、齐墩果酸、白桦脂醇、白桦脂酸)的合成及相关基因的上调表达,且Ca2+的加入使得这种诱导效应更为显著,暗示白桦细胞中钙信号分子介导了水杨酸诱导三萜物质的合成。而对SA与各种Ca2+阻断剂互作处理结果显示,Ca2+抑制剂的加入抑制了SA对细胞中三萜合成的诱导效果,表明Ca2+作为信号分子介导了SA诱导白桦悬浮细胞三萜物质的合成。
To investigate the roles of Ca2＋ as a signal molecule in the salicylic acid （SA）-induced triterpenoids synthesis in suspension cells of Betula platyphylla, accumulation of total triterpenes and its components in white birch suspension cells, treated by SA, Ca2＋ and the calcium channel blocker, were analyzed through UV spectrophotometry and HPLC. Also expression changes of key enzyme genes, such as FPS, SS, SE, BPW, BPY, CAS and calmodulin gene in terpene synthesis pathway of the birch cell were detected by real-time PCR. The results showed that salicylic acid can induce triterpenoid substances （total triterpene, oleanolic acid, betulin, betulinic acid） synthesis and some genes were up-regulated in birch cell suspension. Furthermore the addition of Ca2＋ would enhance the induction effect more significantly, which suggested that calcium signaling molecules in birch ceils mediated the salicylic acidinduced synthesis of triterpenoid substances. In addition the results of SA combined with a variety of Ca2＋ blocker treatment showed that Ca2＋ inhibition would restrain the effect of salicylic acid （SA）-induced triterpenoids synthesis. Taken togetber these results indicated that Ca2＋ as a signal molecule would mediate the synthesis of triterpenoids induced by SA in white birch suspension cells.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2014年第2期51-58,共8页
Journal of Beijing Forestry University
基金
国家自然科学基金项目(31070531
31200428)
黑龙江省自然科学基金项目(C201110)
哈尔滨市青年创新基金项目(2012RFQXN007)
关键词
白桦
三萜物质
关键基因
钙调蛋白
Betula platyphylla
triterpenoid substances
key gene
calmodulin
