摘要
目的 探讨痉挛性喉炎患儿入院时、出院前及出院2个月血清IL-4、γ干扰素（IFN-γ）和IgE水平的变化及其临床意义.方法 采用双抗体夹心酶联免疫吸附法检测50例痉挛性喉炎患儿入院时（急性期）、出院前（临床症状消失期）、出院后随访2个月及30例健康对照组儿童血清IL-4、IFN-γ水平,采用荧光酶联免疫法检测IgE水平,并进行比较.结果 痉挛性喉炎急性期患儿血清IL-4水平显著高于健康对照组[（20.65±5.87） ng/L比（8.23 ±2.71） ng/L,t=5.536,P＜0.05],临床症状消失期较急性期明显下降[（11.07±2.93）ng/L比（20.65±5.87） ng/L,F=5.258,P＜0.05];痉挛性喉炎急性期患儿血清IFN-γ水平显著低于健康对照组[（61.38±6.36） ng/L比（108.42±13.56） ng/L,t=4.831,P＜0.05],临床症状消失期[（95.12±11.63）ng/L]较急性期明显升高（F=4.239,P＜0.05）,与健康对照组比较差异无统计学意义（P＞0.05）,出院2个月[（75.68 ±6.29） ng/L]与健康对照组比较差异无统计学意义（P＞0.05）;痉挛性喉炎急性期患儿血清IgE水平显著高于健康对照组[（136.63±17.86） ng/L比（47.16 ±6.83） ng/L,P＜0.05],症状消失期较急性期[（88.07 ±11.83） μg/L]明显下降（F=5.182,P＜0.05）,但高于健康对照组（P＜0.05）,出院2个月[（86.72 ±7.25） μg/L]仍高于健康对照组（P＜0.05）.结论 痉挛性喉炎患儿存在Th1/Th2功能失衡,其可能是特应性疾病的一种表现形式,IL-4、IFN-γ和IgE在患儿免疫病理机制中起重要作用.
Objective To investigate the clinical significance and changes of serum interleukin-4 （IL-4）,interferon-γ（IFN-γ） and immunoglobulin E （IgE） levels in infants with spasmodic laryngitis.Methods Serum samples were obtained from 50 children with spasmodic laryngitis and 30 healthy children.The changes of IL-4,IFN-γand IgE levels in serum when hospital admission （acute stage）,before discharge （the stage of clinical symptom disappeared） and after 2 months of follow-up were observed.Enzyme-linked immunosorbent assay was used to determine the levels of IL-4 and IFN-γ in serum.Serum IgE level was determined with enzyme-linked fluoroimmuneassay.Results The serum level of IL-4 in patients with spasmodic laryngitis during acute stage were much higher than that in normal control group[（20.65 ±5.87） ng/L vs（8.23 ±2.71） ng/L,t =5.536,P ＜0.05].It was decreased during the stage of clinical symptom disappeared and significantly lower than that during acute stage[（11.07 ± 2.93） ng/L vs （20.65 ± 5.87） ng/L,F =5.258,P ＜ 0.05].The serum level of IFN-γ in patients with spasmodic laryngitis during acute stage were much lower than that in normal control group[（61.38 ±6.36） ng/L vs （108.42 ± 13.56） ng/L,t =4.831,P ＜ 0.05],and it was increased during the stage of clinical symptom disappeared [（95.12 ± 11.63） ng/L] and significantly higher than that during acute stage（F =4.239,P ＜ 0.05）.There was no difference between the patients at clinical symptom disappeared stage and normal control group （P ＞ 0.05）.Two months after discharge,there was no difference in serum INF-γlevel between the patients [（75.68 ±6.29） ng/L] and that in control group （P ＞ 0.05）.The serum level of IgE in patients with spasmodic laryngitis during acute stage were much higher than that in normal control group [（136.63 ± 17.86） ng/L vs （47.16 ± 6.83） ng/L,t =6.685,P ＜ 0.05].It was decreased during the stage of clinical symptom disappeared [（88.07 ± 11.83） μg/L] and significantly lower than that during acute stage（F =5.182,P ＜ 0.05）,but higher than that of normal control group （P ＜ 0.05）.Two months after discharge [（86.72 ± 7.25） μg/L] it was still higher than that in control group （P ＜ 0.05）.Conclusions There is TH1/TH2 imbalance in infants with spasmodic laryngitis.Maybe spasmodic laryngitis is one of the clinical manifestations of atopic diseases.IL-4,IFN-γand IgE may play important roles in immunologic pathogenesis.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2014年第4期286-288,共3页
Chinese Journal of Applied Clinical Pediatrics
