摘要
目的探讨参松养心胶囊对心肌梗死(简称心梗)后神经重构的影响。方法新西兰大耳白兔行冠状动脉结扎术后随机分为心梗组、心梗+参松组(每公斤体重每天0.4 g)。假手术组开胸后在冠状动脉下穿线,但不结扎。术后8周,处死动物,从家兔梗死周边区及非梗死区取材。免疫组化研究生长相关蛋白(GAP43)及酪氨酸羟化酶(TH)阳性纤维的密度。逆转录聚合酶链式反映研究核因子(NF-κB)、内皮素-1(ET-1)、神经生长因子(NGF)、白细胞介-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)及神经营养素受体P75(p75-NTR)mRNA的相对表达量。结果与假手术组(n=10)相比,心梗组(n=8)神经纤维密度显著增加,NF-κB、ET-1、NGF、IL-1β、TNF-α及p75-NTR的mRNA表达显著升高(P<0.05)。与心梗组相比,心梗+参松组(n=9)神经纤维密度减少,NF-κB、ET-1、NGF、IL-1β、TNF-α及p75-NTR mRNA的表达降低(P<0.05)。结论参松养心胶囊可以降低心梗后的神经密度,其作用机制可能与抑制炎症因子及NGF信号途径相关。
Objective To evaluate the effect of shen song yang xin （SSYX）on neural remodeling after myocardial in- farction in rabbits. Methods New Zealand White rabbits were randomly assigned into myocardial infarction （MI）group and MI＋（SSYX） （0.4g/Kg. d-l ） group after successful establishment of model of MI. Sham operation group were not liga- ted in the same part of heart. Eight weeks after treatment, rabbits were sacrificed and samples were obtained from peri-in- farcted and non-infarcted area. Nerve density were assessed by GAP43 and TH immunohistochemical technique. The nuclear transcription factor（ NF-KB）, endothelin-l（ ET-1 ）, nerve growth factor（ NGF）, interleukin-1β （IL-1β ）, tumor necrosis fac- tor- or（TNF-α） and p75-NTR mRNA expression were examined. Results Compared to sham operation group,the densi- ties of immunostaining positive nerve fibers were significantly higher and expressions of NF-KB, ET-1, NGF, IL-1β, TNF-ot and p75-NTR mRNA significantly increased in MI group（ P〈0.05 for all） . Compared to MI group, the densities of GAP43 and TH positive nerve fibers decreased and expression of NF-KB, ET-1, NGF, IL-1β, TNF-α and p75-NTR mRNA were low- ered in MI＋ （SSYX） group （P〈0.05 for all）. Conclusion SSYX inhabits neural remodeling , which may be correlated with the inhabition of proinflammatory response and NGF signaling.
出处
《中国心脏起搏与心电生理杂志》
2014年第1期59-62,共4页
Chinese Journal of Cardiac Pacing and Electrophysiology
基金
国家重点基础研究发展计划(973计划)2012CB518604
关键词
心血管病学
神经重构
参松养心胶囊
心肌梗死
Cardiology
Neural remodeling
Shen song yang xin capsule
Myocardial infarction
