摘要
以短乳杆菌(Lactobacillus brevis)Lb-2菌株cDNA为模板克隆了谷氨酸脱羧酶(Glutamate decarboxylase,GAD)基因。采用在线分析工具及相应软件分析预测了GAD基因核苷酸和氨基酸序列的组成、理化性质、信号肽以及高级结构等,并构建系统发育树。该基因序列全长1 407 bp,为一个完整的阅读框,编码468个氨基酸。GAD相对分子量理论预测值和等电点分别是53 517.8 u和5.42,没有跨膜区,没有其他亚细胞定位序列,为亲水性蛋白,与植物乳杆菌(Lactobacillus plantarum)和德氏乳酸杆菌(Lactobacillus delbrueckii)的GAD进化关系最近。
Glutamate deearboxylase （GAD） gene was cloned using cDNA of LactobaciUus brevis strain Lb-2 as a template and then sequenced. The on-line analysis tools and corresponding softwares the were adopted to analyse the components of nucleotide and amino acid sequences the GAD gene, predict the physical and chemical properties, signal peptide as well as advanced structure, and establish a phylogenetic tree. The gene sequence was a complete open reading frame （ORF） of 1 407 bp coding 468 amino acids. The theoretically relative predicted molecular weight and isoelectric of GAD was 53 517.8 u and 5.42 respectively, without trans-embrane domain, no subcellular localization sequence, and was hydrophilic protein, having the closest evolutionary relationship of GAD with that of Lactobacillus plantarum and L. delbrueckii.
出处
《微生物学杂志》
CAS
CSCD
2014年第1期5-11,共7页
Journal of Microbiology
基金
国家自然科学基金(31070008
31270061)
关键词
短乳杆菌
谷氨酸脱羧酶
生物信息学
基因克隆
LactobaciUus brevis
glutamate decarboxylase （GAD）
bio-infonnatics
gene cloning
