摘要
家蚕是卵滞育性鳞翅目模式昆虫,其滞育卵的活化与生物钟蛋白TIME-EA4密切相关。TIME-EA4具备稳定的Cu/Zn SOD活性和瞬时的ATP酶活性。目前,国内外关于家蚕TIME-EA4的研究主要集中在结构和功能上,其分子进化机制研究尚未开展。本文利用生物信息学方法对TIME-EA4进行了进化踪迹分析,结果显示TIME-EA4的重要氨基酸残基(coverage<25%)的91.2%都用来维持与Cu/Zn SOD的序列一致性,但TIME-EA4与Cu/Zn SOD的Cu/Zn离子结合位点在氨基酸残基组成、极性、绑定原子方面都存在差异。
Bombyx mori is the model insect of Lepioloptera with diapause during the egg stage, and the activation of diapause eggs is closely related to the biological clock protein TIME-EA4. TIME-EA4 has stable Cu/Zn SOD activity and one-time transitory ATPase activity. Numerous studies have focused on the structural and functional characteristics of TIME-EA4. The molecular evolution of this protein has received little attention. In this study, bioinformatics methods were used to analyze the evolutionary trace of TIME-EA4. The results showed that 91.2% of the important amino acid residues in TIME-EA4 were consistent with Cu/Zn SOD. However, there were some differences such as composition, amino acid residue polarity and bonded atoms in Cu/Zn ion binding sites were observed between these proteins.
出处
《四川动物》
CSCD
北大核心
2014年第2期184-189,共6页
Sichuan Journal of Zoology
基金
国家自然科学基金项目"家蚕滞育生物钟蛋白质EA4的分子作用机理研究"(30371086)
国家自然科学基金项目"基于ea4与sod基因互作的滞育生物钟分子调控机制研究"(30771632)
关键词
家蚕
生物钟
生物信息学
分子进化
进化踪迹
Bombyx mori
biological clock
bioinformatics
molecular evolution
evolutionary trace
