摘要
目的：建立地锦喷雾剂的质量标准.方法：采用薄层色谱法鉴别地锦草、丁香、紫花地丁、白鲜皮;采用高效液相色谱法测定芦丁和槲皮素的含量.结果：薄层色谱特征明显,斑点清晰,分离度好;芦丁在6.1031～195.2990μg/ml范围内线性关系良好（r=0.9999,n=6）,平均回收率为98.81％（RSD=1.10％,n=9）;槲皮素在6.1760～197.6320μg/ml范围内线性关系良好（r=1.0000,n=6）,平均回收率为98.35％（RSD=1.02％,n=9）.结论：本文所建立的方法操作方便、结果准确、专属性强,可用于地锦喷雾剂的质量控制.
OBJECTIVE：To estabhsh the quality standard of Euphorbia Humifusa Spray. METHODS：Euphorbiae Humifusae Herba, Caryophylli Flos, Viola Yedoensis and Dictamni Cortex were identified by TLC. The contents of eldrin and quertetin were determined by HPLC. RESULTS：The TLC was characteristic and well-separated with clear spots. Eldrin showed a good linear relationship at a range of 6. 103 1-195. 299 0 μg/ml（ r = 0. 999 9, n = 6 ）with an average recovery of 98.81% （RSD = 1.10% ,n =9） ; quertetin showed a good linear relationship at a range of 6. 176 0-197. 632 0 μg/ml （ r = 1. 000 0, n = 6） with an average recovery of 98.35 % （RSD = 1.02%, n = 9）. CONCLUSION ： The established method is convenient in operation, accurate in results with strong specificity and it thus can be applied for the quality control of Euphorbia Humifusa Spray.
出处
《中国医院用药评价与分析》
2014年第3期228-231,共4页
Evaluation and Analysis of Drug-use in Hospitals of China
基金
济南军区后勤科研计划(编号:CJN12L078)
