摘要
本研究采用GnRH并列体二聚物(GnRH-Tandem-Dimer,GTD)为免疫原主动免疫SD雌鼠,检测下丘脑-垂体-卵巢轴生殖相关基因表达变化,分析主动免疫GTD对SD雌鼠生殖功能的影响。选取48只性成熟SD雌鼠随机分为GTD免疫去势组和空白组。免疫组雌鼠于12周龄时初免GTD免疫去势疫苗,4周后加免。每两周采集血液检测血清激素含量变化。分别于加免后4、8、12周处死大鼠,每组处死8只,收集血液;并采集下丘脑、垂体、卵巢,分析生殖相关基因mRNA表达变化。结果显示,GTD主动免疫后大鼠卵巢严重萎缩,重量下降到对照组的23.12%(p<0.05);血清FSH、LH、P4及E2含量均显著下降(p<0.05);除下丘脑GnRH mRNA先上升后下降外,下丘脑ER、垂体GnRH-R、LH-β、FSH-β和卵巢LH-R、FSH-R和ER mRNA表达水平显著下调(p<0.05)。结果表明,GTD主动免疫可通过对生殖内分泌激素分泌的调节和对生殖相关基因mRNA表达水平的调控,影响下丘脑-垂体-卵巢轴的功能。
The present study was designed to investigate the effect of active immunization against GnRH-Tandem-Dimer （GTD） on functions of hypothalamic-pituitary-ovarian axis in female rats. 48 adult female Sprague-Dawley （SD） rats were randomly allocated to two groups. One group was immunized at age of 12 weeks with a booster vaccination 4 weeks later. The other group were not administrated and served as control group. Blood samples for hormone assays were collected on the day of 1~＇ vaccination and every 2 weeks later on. The rats were decapitated at 4, 8, 12 weeks after booster vaccination （n = 8, respectively）. At autopsy, hypothalamic, pituitary and ovary were collected to detect the mRNA expressions of reproduction-related genes. The results showed that in immunocastrates, the ovaries were atrophied seriously, and weight of which were decreased to 23.12% of controls at 12 weeks of after booster vaccination （p 〈 0.05） ; serum FSH, LH, P4 and E2 levels were significantly decreased to low or non-detectable levels （p 〈 0.05）. In contrast to controls, except hypothalamic GnRH, the mRNA expression levels in hypothalamic ER, pituitary GnRH-R, LH - 13 and FSH - 13 and ovarian LH-R, FSH-R and ER in immunocastrated rats were significantly down-regulated after immunization （p 〈 0.05）. In summary, the functions of hypothalamic-pituitary-ovarian axis would affected by active immunization against GDT through regulating reproductive hormones level and related gene expressions.
出处
《核农学报》
CAS
CSCD
北大核心
2014年第2期335-342,共8页
Journal of Nuclear Agricultural Sciences
基金
四川省科技厅国际合作项目(2012HH0013)
四川农业大学双支计划(00770107)
