摘要
研究了微波辅助提取和田玉枣环磷酸腺苷(cAMP)工艺参数,并与传统溶剂法和超声波辅助法对比。结果表明,微波辅助提取和田玉枣cAMP最优工艺条件为:乙醇体积分数10%,微波功率300 W、微波时间3 min、浸泡时间6 h、料液比1∶30,和田玉枣cAMP提取量为795.63μg/g;各因素影响和田玉枣cAMP提取量的显著顺序:微波时间>微波功率>料液比>浸泡时间;微波辅助法较传统溶剂法和超声波辅助法提取量高、提取时间短、能耗低。采用LS-200型树脂分离纯化和田玉枣cAMP的分离效率为97.81%,cAMP粗品纯度3.47%。
The process parameters for the microwave-assisted extraction of cAMP from Hetianyuzao systemically was investigated in the study,and the results were compared with the traditional solvent extraction and ultrasonic-assisted extraction methods.The processing parameters of extracting cAMP from Hetian jade jujube with microwave-assisted extraction were obtained and listed as follows that：ethanol concentration 10%,microwave-assisted power 300 W,microwave-assisted extraction time 3min,soak period 6 h,liquid/solid ratio 1∶30; Under the optimal extraction conditions,the yield of cAMP was 795.63 μg/（g ·dw）.The effect sequence of four main factors for the yield were as follows：microwave time〉microwave power〉liquid/solid ratio〉soak period time.Microwave-assisted method extracted more cAMP than that of the traditional solvent extraction during short time,and extracted more cAMP than ultrasonic wave-assisted extraction with low energy consumption.The separation efficiency of cAMP by LS-200 was 97.81%,in 3.47% purity.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2014年第3期293-300,共8页
Journal of Food Science and Biotechnology
基金
科技部农业科技成果转化基金项目(2011GB2360)
陕西省科技计划项目(2012JM3014)
陕西省教育厅科研计划项目(12JK0818)
陕西学前师范学院科研基金项目(2013KJ063)
关键词
和田玉枣
环磷酸腺苷
微波
提取
hetian jade jujuba
cAMP
microwave-assisted
extraction
