摘要
采用构建细菌16S rRNA基因文库研究郎酒超高温大曲中细菌群落结构。随机挑选58个阳性克隆子进行测序分析。结果表明,大曲内细菌群落组成较为简单,主要分布于芽孢杆菌科和高温放线菌科;将序列相似度大于或等于97%的定义为同一个OTU(Operational taxonomic units),可分为6个不同的OTUs。其中,枝芽孢杆菌属(Virgibacillus)和高温放线菌属(Thermoactinomyces)为优势种群,占全部克隆子的比例分别为44.83%和43.1%;其他类群包括芽孢杆菌属(Bacillus)和葡萄球菌属(Staphylococcus)。
The structure of bacterial communities of ultrahigh-temperature Langjiu Daqu was studied through the construction of bacteria 16S rRNA gene clone library. 58 positive clones were randomly selected for sequencing analysis. The results showed that, bacterial biodiversity of Daqu was quite simple and mainly distributed in Bacillaceae and Thermoactinomycetaceae, and 58 positive clones could be divided into 60TUs （ the similarity of different sequences higher than or equal to 97 % is defined as the same OTU）. Among them, Virgibacillus and Thermoactinomyces were the dominant groups and they accounted for 44.83 % and 43.1% respectively of total clones. Other groups included Bacillus and Staphylo-
出处
《酿酒科技》
北大核心
2014年第4期5-8,共4页
Liquor-Making Science & Technology
基金
四川省教育厅重大培育项目(09ZZ015)
四川理工学院人才引进项目(2010XJKRL001)
酿酒生物技术及应用四川省重点实验室项目(NJ2010-05
NJ2011-05)
关键词
微生物
白酒
超高温大曲
细菌
群落结构
16S
rRNA基因文库
microbes
Baijiu（liquor）
ultrahigh-temperature Daqu
bacteria
community stucture
16S rRNA gene library
