摘要
目的：测定柿树寄主的桑寄生科7种不同属种的桑寄生药用植物中槲皮苷含量。方法：采用HPLC法对柿树寄主的桑寄生科7种不同属种桑寄生药用植物槲皮苷含量进行测定，样品采用甲醇超声提取方法制备，用Inertsil ODS-SP 色谱柱（150 mm×4.6 mm，5μm），甲醇-0.1%磷酸溶液（52：48）为流动相，流速1.0 mL·min-1，检测波长254 nm。结果：槲皮苷的线性范围0.21-350.00 mg·L-1（ r =0.9999），平均回收率为98.86%，RSD=2.31%。桑寄生科7种不同属种药用植物茎枝中槲皮苷含量为0.3589-3.6770 mg·g-1，叶中槲皮苷含量为5.0091-76.3813 mg·g-1。结论：桑寄生科不同属种桑寄生药用植物槲皮苷的含量各不相同，主要存在于叶中，而茎枝中的含量较低。
This study was aimed to determine contents of quercitrin in 7 Taxilli Herba parasitized in Diospyros kaki Thunb . The content of quercitrin in Taxilli Herba parasitized in D. kaki Thunb. was determined by HPLC and quercitrin was refluxing extracted using methanol. The analytical column was Inertsil ODS-SP （150 mm × 4.6 mm, 5 μm）. The mobile phase was methanol-0.1% phosphate （52：48）. The flow rate was 1.0 mL·min-1. The detection wavelength was 254 nm. The results showed that the linear range of quercetin was within the range of 0.21-350.00 mg·L-1 （r = 0.999 9）. The average recovery rate was 98.86%, RSD = 2.31%. Contents of quercitrin from 7 kinds of Taxilli Herba parasitized were 0.358 9-3.677 0 mg·g-1 in branches and 5.009 1-76.381 3 mg·g-1 in leaves. It was concluded that contents of quercitrin in different kinds of Taxilli Herba parasitized in D. kaki Thunb. are different, which are mainly found in the leaves. The content in branches is relatively low.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第2期368-372,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委面上项目(81173537):寄主植物对桑寄生药材质量影响评价研究
负责人:李永华
广西科学技术厅科技攻关项目(桂科攻11107009-1-12):广西道地药材桑寄生院内制剂的研究与开发
负责人:朱开昕
广西自然科学基金委面上项目(2010GXNSFA013264):寄主植物对桑寄生药材质量影响评价研究
负责人:李永华
关键词
桑寄生
柿树
槲皮苷
HPLC法
Taxilli Herba, Diospyros kaki Thunb., quercitrin, HPLC method
