摘要
目的：建立补阳还五汤挥发性成分的气相色谱-质谱联用（GC-MS）指纹图谱。方法：色谱柱：5HP-MS，载气：He，流速：1.0 mL·min-1，进样量：1μL，分流比：1：20。质谱条件：电子轰击（EI）离子源，辅助线温度：280℃，离子源温度：230℃，四级杆温度：150℃，质量扫描范围：m/z 50-550。通过Chemstation和Nist 05a谱库检索认定共有峰。结果：采用中药色谱指纹图谱相似度评价系统软件（2004 A版）对11批样品的指纹图谱进行了匹配，确定并鉴定了9个共有峰，指纹图谱的相似度均在0.93以上。结论：本文建立的GC-MS指纹图谱特征性及专属性强，可以作为补阳还五汤挥发性成分质量控制的稳定方法。
This study was aimed to establish GC-MS fingerprint of Bu-yang Huan-wu （BYHW） decoction. The 5HP-MS quartz capillary column was used. The temperature program carrier gas was He. The velocity was 1.0 mL·min-1. The injection volume was 1 μL. The split ratio was 1：20. The MS conditions were electron impact （EI） ion source with the transmission line temperature at 280℃, ion source temperature at 230℃, and quadrupole temperature at 150℃. The mass scan range was m/z 50-550. And the results were contrasted with ChemStation and NIST 05a. The results showed that nine common peaks were obtained in the fingerprint of eleven batches of BYHW decoction by peaks matched with the Similarity Evaluation System for Chromatographic Fingerprint （2004 A Version）. The similari-ty of fingerprints was calculated more than 0.93. It was concluded that fingerprinting can be used as a method of quality control and provide evidence for the research of volatile ingredient of BYHW decoction.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第3期560-564,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
河南中医学院博士基金(校级)项目(BSJJ2006-02):补阳还五汤配伍规律与药效物质基础
负责人:龙旭阳
