摘要
目的 探讨放射性核素99mTc EHIDA(邻位二乙基乙酰替苯胺亚氨二醋酸 )在肝移植鼠肝胆显像中的作用。方法 实验动物随机分为正常鼠 (NL ,10只 )、正常鼠肝移植 (NL OLT ,8只 )组。应用单光子发射型计算机断层显像仪SPECT行放射性核素肝胆显像检查。结果 10只NL鼠和 6只无并发症NL OLT鼠的肝脏放射性高峰摄取时间、半排泄时间、肠管显像时间 (min)分别为 (3 0 5±0 31)minvs.(3 6 3± 0 6 5 )min (P <0 0 5 )、(6 6 3± 0 6 6 )minvs. (8 2 5± 0 94)min (P <0 0 5 )、(2 84± 0 35 )minvs.(3 13± 0 2 9)min(P >0 0 5 )。NL OLT鼠的时间放射性曲线和肝胆显像图象与正常鼠相似。 2只移植肝功能不良的NL OLT鼠的放射性高峰摄取时间、半排泄时间和肠管显像时间较其余 6只NL OLT鼠明显延长 ,时间放射性曲线则明显异常 ,且其中 1只鼠有放射性核素泄漏表现。结论 99mTc EHIDA可作为一种动态监测移植肝功能和结构变化的有效方法 ,简便安全无创。
Objective[WT5”BZ] To evaluate the role of radionuclide 99m Tc EHIDA for hepatobiliary imaging in rats with orthotopic liver transplantation (OLT) [WT5”HZ]Methods[WT5”BZ] Male SD rats were divided into 2 groups: normal controls (NL, n =10)and normal rats with OLT (NL OLT, n =8). Dynamic scintigraphy using single photon emission computed tomography (SPECT) was used for hepatobiliary imaging. [WT5”HZ] Results[WT5”BZ] The time of peak activity (t max ) and half peak activity(t 1/2 ) in the liver and intestine (GI) imaging time (min) in NL and 6 uncomplicated NL OLT rats were 3 05±0 31 vs. 3 63±0 65 ( P <0 05), 6 63±0 66 vs. 8 25±0 94 ( P < 0 05) and 2 84±0 35 vs. 3 13±0 29 ( P >0 05), respectively. The time active curves and imaging of NL OLT rats were similar to that in NL rats.The t max and t 1/2 in the liver, GI imaging time and the time active curves in 2 complicated NL OLT rats were significantly different compared with that of NL rats. Radionuclide leak occurred in 1 NL OLT rat.[WT5”HZ] Conclusions [WT5”BZ] Radionuclide 99m Tc EHIDA effectively monitors both structural and functional changes of the liver graft. [WT5”HZ]
出处
《中华普通外科杂志》
CSCD
北大核心
2001年第1期34-36,共3页
Chinese Journal of General Surgery
关键词
肝移植
放射性核素
大鼠
Liver transplantation
Radionuclide
Rats
