摘要
转基因植物中转基因沉默已成为植物基因工程的一大障碍 .转基因沉默的原因是多方面的 ,可能是由于转录前外源基因和内源基因的结构特性、位置效应以及宿主植物的遗传调控 ;也可能是因为转录时启动子、转录因子和终止子的作用 ;还可能是由于转录后的修饰作用、外源基因表达特异性和环境等因素 .为了克服转基因植物中转基因沉默 ,可以采取下列对策 :选择适宜的外源基因和调控元件、采用适当的转化方法。
In the course of commercialization plant gene engineering, transgene silencing is one of obstacles. It can result from structural characters of transgenes and endogens, position effect and genetic regulations of host plants before transcription; it happens due to effects of promoter, transcriptional factor and terminator when transgenes transcript; and it appears in view of modifications of post transcription, specific expression of foreign genes, as well as environmental effects. Thus, we can promote expression levels of transgene by selecting suitable transgene and regulatory elements, using advanced transformational methods and adopting the more concise and quicker means to select transformants.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第1期62-67,共6页
Journal of Southwest China Normal University(Natural Science Edition)
基金
教育部<高等学校骨干教师资助计划>资助项目
