摘要
本研究探讨人急性淋巴母细胞白血病细胞株Molt-4的遗传学特性,评价其在Flow-FISH方法检测细胞端粒长度中的应用价值。悬浮培养Molt-4细胞并进行规律传代,选取8代不同代数、处于对数生长期的Molt-4细胞进行研究。应用细胞计数法描绘细胞生长曲线并计算细胞倍增时间,流式细胞术检测细胞DNA倍体指数,常规G显带进行染色体核型分析,Southern blot检测细胞端粒长度。结果显示:Molt-4细胞的倍增时间为(1.315±0.062)d、DNA倍体指数为(2.085±0.0093)、端粒长度为(32.05±5.27)kb,不同代数之间无显著性差异(P值分别为0.931、0.888和0.935)。染色体核型分析结果显示,Molt-4细胞染色体数量为91-99条,多数为超四倍体,并且具有稳定的、特征性染色体结构异常。结论:Molt-4细胞具有稳定的遗传特性和较长的端粒长度,可作为Flow-FISH的内对照细胞。
This study was aimed to investigate the genetic characteristics of human acute lymphoblastic leukemia cell line Molt-4, and evaluate its application in measuring telomere length by Flow-FISH. Molt-4 cell line was cultured in suspension and subcultured regularly. Eight different passages of Molt-4 cells in exponential stage were selected. The growth curves were drawn by cell counting method, meanwhile calculating the population doubling times of cells, DNA ploidies were determined by flow cytometry, karyotypes were analyzed by G-banding and telomere lengths were measured by Southern blot. The results showed that the population doubling time of Molt-4 cell line was （ 1.315 ± 0. 062 ） d, DNA ploidy index was （2. 085 ± 0. 0093 ）, and the telomere length was （32. 05 ± 5.27 ）kb. There were no significant difference among different passages （ P = 0. 931,0. 888 and 0. 935 separately ）. The karyotypes showed that the chromosome numbers of Molt-4 cell line were from 91 to 99 in different metaphases, and the majority of them were hypertetraploid, and stable and recurrent structural abnormalities of chromosomes could be kept. It is concluded that the stable genetic characteristics and the longer telomere length of Molt-4 cell line makes it be a feasible control cells in measurement of telomere length by Flow-FISH.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2014年第2期280-284,共5页
Journal of Experimental Hematology
基金
国家自然科学基金资助项目(81000200)
北京市卫生系统高层次卫生技术人才培养项目(2011-3-092)
