摘要
由糖基转移酶催化产生的次生代谢物质对植物抵御和适应环境的变化起着重要作用。为探讨青花菜在酸雨胁迫下糖基转移酶的表达变化,对青花菜β-1,4-木糖基转移酶IRX9H基因的cDNA序列全长进行克隆,并对其进行生物信息学和表达分析。结果表明:青花菜β-1,4-木糖基转移酶IRX9H基因的cDNA全长为1 550 bp,开放阅读框为1 155 bp,编码384个氨基酸,推测分子式为C1964H3044N558O567S11,分子量为43 897.8,没有信号肽。系统进化树分析结果表明,该青花菜基因与拟南芥聚类关系最近。利用实时荧光定量PCR研究模拟酸雨胁迫下β-1,4-木糖基转移酶IRX9H基因的表达量,结果显示该基因的表达量在模拟酸雨胁迫初期显著增大,随着时间的延长,又开始下降,这表明其在青花菜抗酸雨胁迫中发挥了作用。
Secondary metabolites catalyzed by glycosyltransferase played an important role in resisting and adapting to environmental changes for plants. The full-length cDNA of β-1,4-xylosyltransferase IRX9H was cloned in order to explore its expression in broccoli under acid rain stress, which was analyzed by bioinformatics method and real-time PCR. The results showed that the full-length DNA was 1 550 bp and the opening reading frame（ORF） was 1 155 bp. The ORF encoded 384 amino acids, a deduced formula of C1964H3044N558O567S11 with molecular weight 43 897.8, but no signal peptide. Phylogenetic tree analysis revealed that the gene of broccoli had closer relationship with that from Arabidopsis. The analysis by real-time PCR suggested that the expression of β-1,4- xylosyltransferase IRX9H genes in broccoli under simulated acid rain stress increased significantly at first, and then declined due to longer duration of simulated acid rain stress, which was involved in signalling pathways in response to the acid rain stress.
出处
《热带作物学报》
CSCD
北大核心
2014年第4期729-737,共9页
Chinese Journal of Tropical Crops
基金
福建省大宗蔬菜产业体系
福建省自然基金(No.2012J01082)
福建省蔬菜重大专项
福建农林大学校重点建设项目(No.6112c0409)
