摘要
目的建立测定注射用双黄连（冻干）中果糖、葡萄糖、蔗糖和肌醇含量的HPLC-ELSD方法。方法采用Alltech GRACE prevail carbohydrate ES色谱柱;漂移管温度：100℃;载气（N2）流速：2.0L·min-1;流动相：乙腈-水（7525）;流速：1.0mL·min-1;柱温：30℃。结果果糖、葡萄糖、蔗糖和肌醇进样量分别在0.530~3.975μg（r=0.9996）、0.626~4.695μg（r=0.9999）、0.722~5.415μg（r=0.9997）、0.208~1.560μg（r=0.9995）范围内,质量对数与峰面积对数呈良好的线性关系;平均加样回收率分别为100.1%、97.1%、98.5%、96.1%,RSD分别为2.8%、3.0%、3.4%、3.7%。结论该方法可同时测定注射用双黄连（冻干）中4种糖类成分的含量,分离度好,快速、简便,重现性好,可用于双黄连注射剂中糖类成分的含量测定。
Objective To establish a method for determining the content of D-fructose, D-glucose, sucrose and inositol in Shuanghuanglian powder （lyophilized） for injection by HPLC-ELSD. Methods A chromatographic column of Alltech GRACE prevail carbohydrate ES was adopted. The drift tube temperature was taken at 100 ℃, the carrier gas flow rate at 2.0 L · min-1. The mobile phase was the mixture of acetonitrile-water （75 ： 25） at a flow rate of 1.0 mL · min-1 with the column temperature set at 30 ℃. Results The linear ranges of D-fructose, D-glucose, sucrose and inositol were 0. 530-3. 975 μg （r= 0.9996）, 0.626-4.695 μg （r= 0.9999）, 0.722-5.415 μg （r： 0.9997）, 0.208-1.560 μg （r= 0.9995） respectirely. The average recoveries （n：6） were 100. 1%, 97. 1%, 98. 5%, 96.1% and RSDs were 2.8%, 3.0%, 3.4%, 3.7%. Conclusion The method is simple, accurate, and reliable and can be used for the quantitative determination of the four carbohydrates in Shuanghuanglian powder （lyophilized） for injection.
出处
《中国药事》
CAS
2014年第4期384-387,共4页
Chinese Pharmaceutical Affairs
