摘要
目的 分析载银二氧化钛（TiO2）抗菌涂层气管插管导管的抗菌性能,并确定最低有效抗菌剂浓度.方法 以聚乙烯气管导管为基材,载银TiO2为抗菌剂,经溶胶凝胶方法制备不同浓度的抗菌涂层气管导管,按浓度梯度分为10.0％组、5.0％组、2.0％组、1.5％组、1.0％组、0.8％组、0.6％组、0.2％组和空白组,分别浸渍于1.0×10^5 cfu/mL标准菌株浓度的铜绿假单胞菌、金黄色葡萄球菌、大肠埃希菌菌液中,连同菌液组共10组,过夜24 h,移取10 μL浸渍液在血琼脂培养基上涂布接种,35℃过夜培养后,计数菌落数,以显示其抗菌性能.结果 1.0×10^5 cfu/mL标准菌株浓度条件下,抗菌剂浓度≥1.0％时3种细菌菌落未见明显生长,达到98％以上的杀菌率,表现出了几乎相同的强抗菌效果,组间比较无差异（均P＞0.05）;随抗菌剂浓度降低,3种细菌菌落均呈增加趋势;0.8％组抗菌效果表现一般,与1.0％组和0.6％组比较差异有统计学意义[铜绿假单胞菌（个）：7.300（4.050,8.350）比0.200（0.050,1.200）、9.700（9.000,10.000）,金黄色葡萄球菌（个）：4.100（3.300,4.650）比0.000（0.000,0A50）、5.800（5.350,7.650）,大肠埃希菌（个）：1.400（0.750,3.750）比0.050 （0.025,0.050）、9 500（8.500,9.800）,均P＜0.01].结论 载银TiO2抗菌涂层气管插管导管具有明确的抗菌作用,抗菌性能与抗菌剂浓度有关,浓度在1.0％以上时杀菌性能处于饱和状态,可几乎完全杀灭所有浸渍液中的细菌.
Objective To study the antibacterial property of silver loaded titanium dioxide （TiO2） antibacterial coated endotracheal intubation tube,and to determine the minimum effective antibacterial concentration.Methods Intubation tubes coated with different concentrations of antibacterial agents were prepared with sol gel method.Polyethylene endotracheal intubation tubes were used as substrate,and silver loaded TiO2 was used as the antibacterial agent.According to the different antibacterial concentrations of the antibacterial agent,the tubes were divided into nine groups：10.0% group,5.0% group,2.0% group,1.5% group,1.0% group,0.8% group,0.6% group,0.2% group,and conrol group.They were respectively immersed in three standard bacteria suspensions with 1.0 × l05 cfu/mL：Pseudomonas aeruginosa,Staphylococcus aureus,and Escherichia coll Together with standard bacteria liquid group,there were 10 experimental groups.They were kept overnight for 24 hours.10 μL of respective culture medium was smeared on blood agar culture medium.After being cultured overnight in 35 ℃,the number of bacteria colonies was respectively counted.Results In 1.0 × 10^5 cfu/mL of three standard bacteria liquids with antibacterial agent concentration≥ 1.0%,three bacterial colonies had un-obviously growth rate.Almost the same strong antibacterial effects to achieve sterilizing rates of more than 98% was shown in each group of the antibacterial coating endotracheal intubation tubes （all P〉0.05）.As the antibacterial agent concentration decreased,three bacterial colonies were increasing gradually.Intermediate antibacterial effects were shown in tubes of 0.8% group,with significant statistic difference as compared with 1.0% and 0.6% groups [Pseudomonas aeruginosa：7.300 （4.050,8.350） vs.0.200 （0.050,1.200）,9.700 （9.000,10.000）; Staphylococcus aureus：4.100 （3.300,4.650） vs.0.000 （0.000,0.150）,5.800 （5.350,7.650）; Escherichia coli：1.400 （0.750,3.750） vs.0.050 （0.025,0.050）,9.500 （8.500,9.800）,all P〈0.01].Conclusions Silver loaded TiO2 antibacterial coated endotracheal intubation tube had definite antibacterial properties,which were related to the antibacterial concentration.Strong antibacterial effects were shown when antibacterial concentration was above 1.0%,with bacteria almost completely killed in the immersing liquid.
出处
《中华危重病急救医学》
CAS
CSCD
北大核心
2014年第5期343-346,共4页
Chinese Critical Care Medicine
基金
浙江省医药卫生科技计划项目（2012KYB142）
国家发明专利（ZL2011 1 0361576.7）
关键词
载银二氧化钛
气管插管导管
抗菌涂层
抗菌性能
Silver loaded titanium dioxide
Endotracheal intubation tube
Antibacterial coating
Antibacterial property
