摘要
目的观察吸入2%氢气对大鼠失血性休克复苏诱导的急性肺损伤的影响。方法 24只SD大鼠随机均分为三组:假手术组(S组)、休克组(C组)、H2组(H组)。C组和H组通过缺血60min再灌注120min制作大鼠压力控制型失血性休克复苏模型;S组只进行气管插管、股动静脉穿刺;C组全程吸入50%O2-50%N2混合气,H组灌注开始前10min改为吸入2%H2-48%N2-50%O2混合气至灌注结束。于放血前(T0)、放血15min(T1)、休克60min(T2)、复苏15min(T3)、再灌注60min(T4)和120min(T5)时记录MAP,并于T0、T2和T5时采集动脉血行血气分析。实验结束时取血和肺组织标本,检测肺湿干比(W/D)、核转录因子(NF-κB)p65的表达、血浆中TNF-α水平及肺组织病理学变化。结果不同时点三组大鼠MAP差异无统计学意义。与T0时比较,T2时C组和H组PaO2、PaCO2;T5时C组PaO2和C组、H组PaCO2明显降低(P<0.05)。与S组比较,T2时C组和H组PaO2、PaCO2;T5时C组PaO2和H组PaCO2明显降低(P<0.05)。与C组比较,T5时H组PaO2明显升高(P<0.05),H组肺泡腔炎性细胞浸润、肺泡壁充血及水肿减轻,肺实变组织减少。与C组比较,H组血浆TNF-α和IL-6降低,肺组织W/D、损伤评分和NF-κBp65评分明显降低(P<0.05)。结论吸入2%H2可减轻大鼠失血性休克复苏诱导的急性肺损伤。
Objective To observe the effect of 2% hydrogen on acute lung injury induced by hemorrhagic shock and resuscitation in rats. Methods Twenty-four adult male Sprague-Dawley rats were equally randomized into three groups： sham operation group （group S）, shock group （group C） and hydrogen group （group H）. Rats in the group C and group H were subjected to 60 min of ischemia and 120 min of reperfusion to establish pressure-controlled hemorrhagic shock and resuscitation model. The rats in the group S just received intubation and vascular catheter, The rats in the group C received the mixture of 50% nitrogen-50% oxygen throughout the experiment. The rats in the group H inhaled the mixture of 20% hydrogen-48% nitrogen-50% oxygen 10 min before reperfusion. The MAP was monitored at six time points： before exsanguinations （T0）, 15 min after exsanguinations （T1）, 60 min after shock （T2）, 15 min after resuscitation （T3）, and 60 min （T4） and 120 min （T5） after reperfusion. Arterial blood samples were collected for blood gas analysis at T0, T2 and Ts. Blood and lung tissues were collected at the end of experiment. The plasma levels of pro-inflammatory cytokines （IL-6 and TNF-α） were determined. Lung tissues were assayed for wet/dry weight ratio （W/D）, nuclear transcription factor （NF-κB） p65 expression, and histological changes. Results Compared with T0, PaO2 and PaCO2 at T2 in groups C and H, PaCh at T5 in group C , PaCO2 at T5 in group H were decreased significantly（P〈0.05）. Compared with group S, PaO2 and PaCO2 at T2 in groups C and H, PaO2 in group C and PaCO2 in group H at T5 were decreased significantly（P〈0. 05）. Compared with group C, PaO2 at T5 in group H were increased significantly（P〈 0.05）, there were less infiltration of inflammatory cells ,alveolar wall congestion and edema and consolidation of lung tissue. Compared with group C, TNF-α and IL-6, W/D ratio, injury and NF-kBp65 scores were decreased significantly（P〈0.05）. Conclusion 2% hydrogen inhalation can alleviate acute lung injury in rats with hemorrhagic shock and resuscitation.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2014年第5期490-493,共4页
Journal of Clinical Anesthesiology
基金
黑龙江省自然基金课题(D201109)
关键词
氢气
失血性休克
复苏
急性肺损伤
炎症反应
Hydrogen
Hemorrhagic shock
Resuscitation
Acute lung injury
Inflammatory response
